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预防医学  2017, Vol. 29 Issue (11): 1098-1103    DOI: 10.19485/j.cnki.issn1007-0931.2017.11.005
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小檗碱对溃疡性结肠炎小鼠结肠组织紧密连接蛋白claudin-1表达的影响
沈雁1, 李思2, 钟继红1, 徐磊1, 王章流1, 郑华君1
1.浙江中医药大学附属第二医院消化内科,浙江 杭州 310005;
2.浙江省立同德医院
Effects of berberine extracted from Chinese Goldthread Rhizome on the expression of claudin-1 in ulcerative colitis mice
SHEN Yan, LI Si, ZHONG Ji-hong, XU Lei, WANG Zhang-liu, ZHENG Hua-jun
Department of Gastroenterology,the Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,Zhejiang,310005,China
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摘要 目的 观察黄连提取物小檗碱(BBR)对溃疡性结肠炎(UC)模型小鼠结肠组织claudin-1蛋白及mRNA表达的影响。方法 采用右旋葡聚糖硫酸钠(DSS)建立UC小鼠模型,将60只BALB/c小鼠随机分为空白对照组、模型对照组、BBR低剂量(50 mg/kg)组、BBR高剂量(100 mg/kg)组和柳氮磺吡啶(30 mg/kg SASP)阳性对照组。观察小鼠精神状况、体重等一般情况;评估疾病活动指数(DAI)、结肠大体形态损伤指数(CMDI)和结肠组织损伤指数(TDI);苏木精-伊红染色观察结肠组织的病理学变化;免疫组化SP法和RT-PCR法检测claudin-1蛋白及mRNA表达。结果 与模型对照组相比,各治疗组小鼠结肠炎的临床表现和组织病理表现均有不同程度改善,其中BBR高剂量组和阳性对照组的DAI、CMDI和TDI评分均下降(P<0.01)。5组小鼠claudin-1蛋白mRNA相对表达量分别为1.00±0.03、0.12±0.02、0.21±0.02、0.63±0.03和0.81±0.03。模型对照组和各治疗组小鼠claudin-1蛋白和mRNA表达水平均较空白对照组小鼠降低(P<0.01),经BBR和SASP治疗后表达水平均较模型对照组升高(P<0.01),但BBR低、高剂量组claudin-1 蛋白和mRNA的表达水平均低于阳性对照组(P<0.01)。结论 BBR能够通过上调紧密连接蛋白claudin-1的表达,有效缓解结肠炎症,但其治疗效果较SASP差。
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沈雁
李思
钟继红
徐磊
王章流
郑华君
关键词 小檗碱溃疡性结肠炎claudin-1    
AbstractObjective To investigate the effect of berberine(BBR) extracted from Chinese Goldthread Rhizome on the expression of claudin-1 in ulcerative colitis mice. Methods UC model was established by dextran sulphate sodium(DSS). Sixty BALB/c mice were randomly divided into a blank control group,a model control group,a low dose BBR group,a high dose BBR group and a salazosulfapyridine(SASP)positive control group. Then daily gavage administration was given for 7 days. During the course of experiment,the general conditions of mice were observed,and disease activity index(DAI)were assessed. At the end of treatment,colon tissue were dissected,its macroscopic damage index(CMDI)and tissue damage index(TDI)were evaluated,and the contents of claudin-1 protein and mRNA were detected by immunohistochemical method and real-time PCR separately. Results Compared with model control group,the clinical and pathological symptoms of colitis in BBR groups and SASP group were improved to various degrees,the DAI,CMDI and TDI in high dose BBR group and SASP group were all reduced(P<0.01). The expression levels of claudin-1 mRNA in five groups were 1.00±0.03、0.12±0.02、0.21±0.02、0.63±0.03 and 0.81±0.03,respectively. The expression levels of claudin-1 and its mRNA of colonic tissue in UC mice were all markedly lower than normal mice (P<0.01),which were all increased after BBR or SASP treatment when comparing with blank control group,whose difference was statistically significant. Conclusion Berberine can effectively relieve the colitis in UC mice by raising the expression of claudin-1, but the therapeutic effect was a little weaker than SASP.
Key wordsBerberine    Ulcerative colitis    Claudin-1
收稿日期: 2017-05-08      修回日期: 2017-06-15     
中图分类号:  R285.5  
基金资助:浙江省中医药科技计划项目(2015ZB066); 浙江省医药卫生科技计划项目(2016KYB223)
通信作者: 李思,E-mail:lisi31@163.com   
作者简介: 沈雁,硕士,主治中医师,主要从事消化内科临床工作
引用本文:   
沈雁, 李思, 钟继红, 徐磊, 王章流, 郑华君. 小檗碱对溃疡性结肠炎小鼠结肠组织紧密连接蛋白claudin-1表达的影响[J]. 预防医学, 2017, 29(11): 1098-1103.
SHEN Yan, LI Si, ZHONG Ji-hong, XU Lei, WANG Zhang-liu, ZHENG Hua-jun. Effects of berberine extracted from Chinese Goldthread Rhizome on the expression of claudin-1 in ulcerative colitis mice. Preventive Medicine, 2017, 29(11): 1098-1103.
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