HIV-1 molecular transmission network among men who have sex with men in Shaoxing City
HE Tingting1, CAO Dongqing1, LIN Jiafeng1, JIANG Zhuojing1, ZHANG Jiafeng2, FAN Qin2
1. Department of Microbiological Inspection, Shaoxing Center for Disease Control and Prevention, Shaoxing, Zhejiang 312071, China; 2. Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China
Abstract：Objective To investigate the molecular transmission network characteristics of HIV-1 among men who have sex with men (MSM) in Shaoxing City, Zhejiang Province, so as to provide insights into AIDS prevention and control among MSM. Methods Newly reported HIV/AIDS cases among MSM in Shaoxing City were selected from January 2021 to September 2023. Plasma samples before the antiviral treatment were collected. The HIV-1 pol gene was amplified using reverse transcription PCR and nested PCR to construct phylogenetic trees for gene subtype analysis. The HIV-TRACE method was used to construct a molecular transmission network with a genetic distance of 1.5% to analyze clustering and the characteristics of cases within molecular clusters. Results A total of 216 HIV/AIDS cases among MSM were included, and 179 qualified sequences were obtained. The predominant HIV-1 subtypes were CRF07_BC and CRF01_AE, with 95 and 66 cases, respectively. At 1.5% genetic distance, 20 molecular clusters were formed, with 61 nodes and 58 edges. A total of 61 sequences were connected to the transmission network (34.08%). HIV/AIDS cases among MSM from all the counties (cities, districts) in Shaoxing City were included in the network. There was the largest molecular cluster with ≥10 nodes, involving 12 cases from five counties (cities, districts), 3 medium-sized molecular clusters with 4 to 5 nodes, and the 16 small-sized molecular clusters with 2 or 3 nodes. Seven cases with high risk of transmission, each with ≥4 edges, were all CRF07_BC subtypes. Among them, two cases were from the large molecular cluster, and five cases were from the same molecular cluster composed of cases from Shengzhou City and Xinchang County. Conclusions The predominant HIV-1 subtypes among MSM in Shaoxing City were CRF07_BC and CRF01_AE. There was cross-regional HIV transmission, and potential transmission risk might exist in Shengzhou City and Xinchang County.
何婷婷, 曹栋卿, 林家锋, 蒋卓婧, 张佳峰, 范钦. 绍兴市MSM人群HIV-1分子传播网络特征分析[J]. 预防医学, 2024, 36(7): 571-575,579.
HE Tingting, CAO Dongqing, LIN Jiafeng, JIANG Zhuojing, ZHANG Jiafeng, FAN Qin. HIV-1 molecular transmission network among men who have sex with men in Shaoxing City. Preventive Medicine, 2024, 36(7): 571-575,579.
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