Abstract：Objective To investigate the prevalence and molecular characteristics of Shiga toxin-producing Escherichia coli (STEC) in a large dairy farm and market-sold raw livestock meats in Suzhou City, so as to provide the evidence for evaluating human health risks of STEC. Methods Bovine stool samples and breeding environmental samples were collected from a large dairy farm in Suzhou City, and beef, pork and mutton samples were collected from markets in Suzhou City. STEC strains were isolated and virulence genes were characterized in STEC strains using quantitative fluorescence PCR assay. The sensitivity to common antibiotics was tested using the broth microdilution plate method, and the genotypes of STEC were characterized using pulsed-field gel electrophoresis (PFGE). Results A total of 624 samples were collected, including 110 adult cow stool samples, 170 calf stool samples, 60 farm environmental samples, 126 beef samples, 100 minced beef samples, 15 pork samples, 15 minced pork samples, 18 mutton samples and 10 ground mutton samples. A total of 12 non-O157 STEC strains were isolated, with a detection rate of 1.92%, and the detection rates of non-O157 STEC strains were 4.12%, 1.59% and 3.00% in calf stool samples, beef samples and minced beef samples, respectively, while non-O157 STEC strains were not detected in adult cow stool samples, environmental samples, pork samples, minced pork samples, mutton samples, or minced mutton samples. Among the 12 STEC strains, there were 4 strains carrying stx1 gene, 4 strains carrying stx2 gene and 4 strains carrying stx1 and stx2 genes. The 12 STEC strains showed the highest prevalence of resistance to ampicillin, ampicillin/sulbactam, cefotaxime and cefazoline (all were 41.67%), and were sensitive to imithiomycin, polymyxin, azithromycin, cefoxitin and ciprofloxacin, and there were 5 strains with multidrug resistance (41.67%). The 12 STEC strains were characterized with 11 genotypes and had no unique gene fingerprint patterns, with the Dice similarity coefficient ranging from 61.3% to 92.7%. Conclusions The detection of non-O157 STEC strains is high in calf stools, and non-O157 STEC strains show a level of resistance to common antibiotics and present molecular polymorphisms. The monitoring and management of STEC strains should be strengthened.
黄飞飞, 王波, 张宁, 王小龙, 邹文燕. 苏州市某奶牛养殖场及市售生畜肉产志贺毒素大肠埃希菌分子流行病学分析[J]. 预防医学, 2022, 34(10): 1031-1037.
HUANG Feifei, WANG Bo, ZHANG Ning, WANG Xiaolong, ZOU Wenyan. Molecular characteristics of Shiga toxin-producing Escherichia coli in a dairy farm and market-sold raw livestock meats in Suzhou City. Preventive Medicine, 2022, 34(10): 1031-1037.
[1] MURRAY K F,PATTERSON K,et al.Escherichia coli 0157:H7-induced hemolytic-uremic syndrome:histopathologic changes in the colon over time[J].Pediatr Deve Pathol,2019,3(3):232-239. [2] 白莉. 联合国粮农组织/世界卫生组织关于牛肉和生乳产品生产加工过程中产志贺毒素大肠埃希菌控制措施专家组会议进展[J].中国食品卫生杂志,2020,32(5):589-591. BAI L.Progress of FAO/WHO Expert Group meeting on Control measures for Shiga toxin Escherichia coli in beef and raw milk production and processing.[J].Chin J Food Hyg,2020,32(5):589-591. [3] 邵纯纯. 不同来源产志贺毒素大肠埃希菌的分子流行病学研究[D].济南:山东大学,2017. SHAO C C.Molecular epidemiology research of Shiga toxin-producing E.coli isolated from different sources[D].Jinan:Shandong University,2017. [4] ADAMU M S,UGOCHUKWU I C I,IDOKO S I,et al.Virulent gene profile and antibiotic susceptibility pattern of Shiga toxin-producing Escherichia coli(STEC)from cattle and camels in Maiduguri,North-Eastern Nigeria[J].Trop Anim Health Prod,2018,50(6):1327-1341. [5] 卢丽英,詹丽杏.大肠埃希菌O157︰H7抗生素耐药性研究进展[J].预防医学,2021,33(11):1117-1121. LU L Y,ZHAN L X.Research progress on antibiotic resistance of Escherichia coli O157︰H7[J].Prev Med,2021,33(11):1117-1121. [6] 2019年STEC风险评估项目江苏省工作方案[Z].2019:8-15. [7] NEOH H M,TAN X E,SAPRI H F,et al.Pulsed-field gel electrophoresis(PFGE):a review of the "gold standard" for bacteria typing and current alternatives[J/OL].Infect Genet Evol,2019,74[2022-07-05].https://doi.org/10.1016/j.meegid.2019.103935. [8] Clinical and Laboratory Standards Institute.Performance standards for antimicrobial susceptibility testing;twentieth informational supplement[M].Wayne:Clinical and Laboratory Standards Institute,2016. [9] 薛涛,李丽,高清清,等.牛源产志贺毒素大肠杆菌分离株的毒力基因分布和遗传进化分析[J].中国畜牧兽医,2017,45(10):2878-2885. XUE T,LI L,GAO Q Q,et al.Study on the virulence gene distribution and genetic evolution of cattle Shiga toxin-producing Escherichia coli isolates[J].China Anim Husbandry Vet Med,2017,45(10):2878-2885. [10] 巴鹏斌,孟琼,白向宁,等.非O157产志贺毒素大肠埃希菌研究进展[J].中国人兽共患病学报,2017,33(2):156-162. BA P B,MENG Q,BAI X N,et al.Research progress on non-O157 Shiga toxin-producing Escherichia coli[J].Chin J Zoonoses,2017,33(2):156-162. [11] DE ASSIS D C S,DA SILVA T M L,BRITO R F,et al.Shiga toxin-producing Escherichia coli(STEC)in bovine meat and meat products over the last 15 years in Brazil:a systematic review and meta-analysis[J/OL].Meat Sci,2020,173[2022-07-05].https://doi.org/10.1016/j.meatsci.2020.108394. [12] BRUSA V,ALIVERTI V,ALIVERTI F,et al.Shiga toxin-producing Escherichia coli in beef retail markets from Argentina[J/OL].Front Cell Infect Microbiol,2013[2022-07-05].https://doi.org/10.3389/fcimb. [13] BARANZONI G M,FRATAMICO P M,RUBIO F,et al.Detection and isolation of Shiga toxin-producing Escherichia coli(STEC)O104 from sprouts[J].Int J Food Microbiol,2014,173:99-104. [14] HINES J Z,BANCROFT J,POWELL M,et al.Case finding using syndromic surveillance data during an outbreak of Shiga toxin-producing Escherichia coli O26 infections,Oregon,2015[J/OL].Public Health Reports,2017,132(4)[2022-07-05].https://doi.org/10.1177/0033354917708994. [15] 胡彬,寇增强,邵纯纯,等.山东省动物粪便中非O157产志贺毒素大肠埃希菌菌株特征及耐药性分析[J].中华预防医学杂志,2018,52(3):271-276. HU B,KOU Z Q,SHAO C C,et al.Characteristics and drug resistance of non-O157 Shiga toxin-producing E.coli in animal feces from Shandong Province[J].Chin J Prev Med,2018,52(3):271-276. [16] COBBOLD R,DESMARCHELIER P.A longitudinal study of Shiga-toxigenic Escherichia coli(STEC)prevalence in three Australian dairy herds[J].Vet Microbiol,2000,71(1/2):125-137. [17] CERGOLE-NOVELLA M C,PIGNATARI A C,CASTANHEIRA M,et al.Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains(STEC)in Brazil[J].Res Microbiol,2011,162(2):117-123. [18] 吴少鹏,王国华,赵效南,等.山东省某地区奶牛源大肠埃希菌的血清型、耐药特性及分子特性[J].微生物学报,2020,60(3):486-498. WU S P,WANG G H,ZHAO X N,et al.Serotyping,drug resistance and molecular characteristics of Escherichia coli from dairy cows in a region of Shandong province[J].Acta Microbiol Sin,2020,60(3):486-498. [19] 郑晓风,张妍,刘英玉,等.新疆部分地区牛羊源产志贺毒素大肠埃希茵菌株检测与分析[J].畜牧兽医学报,2020,51(10):2518-2527. ZHENG X F,ZHANG Y,LIU Y Y,et al.Detection and analysis of Shiga toxin-producing Escherichia coli isolates fromcattle and sheep sources in some regions of Xinjiang,China[J].Chin J Anim Vet Sci,2020,51(10):2518-2527. [20] CIMOLAI N,CARTER J E,MORRISON B J,et al.Risk factors for the progression of Escherichia coli O157∶H7 enteritis to hemolytic-uremic syndrome[J].J Pediatr,1990,116(4):589-592. [21] KAKOULLIS L,PAPACHRISTODOULOU E,CHRA P,et al.Shiga toxin-induced haemolytic uraemic syndrome and the role of antibiotics:a global overview[J].J Infect,2019,72(2):75-94. [22] LAUNDERS N, BYRNE L, JENKINS C,et al.Disease severity of Shiga toxin-producing E.coli O157 and factors influencing the development of typical haemolytic uraemic syndrome:a retrospective cohort study,2009-2012[J/OL].BMJ Open,2016,6(1)[2022-07-05].http://dx.doi.org/10.1136/bmjopen-2015-009933. [23] 李伟,崔志刚,阚飙,等.转化预防医学的实践:PulseNet China[J].疾病监测,2012,27(2):88-92. LI W,CUI Z G,KAN B,et al.Practice on translational preventive medicine:PulseNet China[J].Dis Surveill,2012,27(2):88-92.
