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预防医学  2016, Vol. 28 Issue (3): 234-239    
  论著 本期目录 | 过刊浏览 | 高级检索 |
二氯甲烷重复剂量经皮染毒对大鼠毒作用的研究
孟真, 陈苘, 吴惠岭, 夏勇, 傅剑云, 张世鑫, 朱周靓
浙江省疾病预防控制中心,浙江 杭州 310051
A study on the effect of repeated-dose toxicity on rats exposed to dichloromethane by dermal route for 28 days
MENG Zhen, CHEN Qing, WU Hui-ling, XIA Yong, FU Jian-yun, ZHANG Shi-xin, ZHU Zhou-jin
The Center for Disease Control and Prevention of Zhejiang Province, Hangzhou, Zhejiang,310051, China
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摘要 目的 观察28 d经皮给予较高剂量二氯甲烷后SD大鼠的毒性反应。方法 实验设1个溶剂对照组(橄榄油)、4个二氯甲烷染毒组(0.75、1.5、3.0和6.0 g/kg·BW)和1个恢复组(6.0 g/kg·BW染毒后恢复2周),每组各取12只大鼠观察,雌雄各半。其中二氯甲烷染毒组大鼠每天经皮染毒6 h,每周5 d,连续4周。比较动物间体重、进食等一般状况的变化和试验期满血常规、血生化指标检测结果及主要脏器病理改变。结果 试验期内雄鼠体重增长缓慢。血常规检测发现二氯甲烷染毒3.0和6.0 g/kg组雌鼠和1.5、3.0、6.0 g/kg组雄鼠淋巴细胞减少和中性粒细胞增多,与溶剂对照组比较差异有统计学意义(P<0.05)。染毒3.0和6.0 g/kg组雄鼠血清谷丙转氨酶和谷草转氨酶水平升高,6.0 g/kg组雄鼠血清甘油三酯含量降低,与溶剂对照组比较差异有统计学意义(P<0.05);而雌鼠未见明显异常。病理学结果显示大鼠染毒区皮肤表皮层和真皮层出现病变,肝细胞变性和坏死等病变,具有剂量-效应关系。恢复组大鼠在恢复期内血清生化指标和白细胞分类恢复正常,染毒区局部皮肤病变和肝细胞损害有一定程度改善,但未完全恢复。结论 二氯甲烷28 d经皮肤重复剂量染毒可引起大鼠染毒区局部皮肤和肝细胞损伤,并伴随雄鼠体重、部分血常规及血清生化指标改变。
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孟真
陈苘
吴惠岭
夏勇
傅剑云
张世鑫
朱周靓
关键词 二氯甲烷大鼠重复剂量毒性皮肤病变肝脏损伤    
AbstractObjective To learn the toxic effects on rats repeatedly exposed to dichloromethane by dermal route at the same dose for 28 days. Methods Seventy two Sprague Dawley rats were divided into six groups: a solvent control group (oliver oil), four exposure groups (0.75,1.5,3.0 and 6.0 g/kg·BW) and a recovery group. Each group included six female and six male rats.The exposure groups were dermal exposed to DCM at 1.5, 3.0 and 6.0 g/kg·BW, 6 hours/ day, 5 days/ week for four weeks. The recovery group was dermal exposed to 6.0 g/kg BW of DCM during the same period then recovered for 2 weeks. Clinical signs including body weight and food consumption of each group were observed. After rats were sacrificed, the hematological and serum biochemical parameters were determined. Histopathological examination was performed on selected tissues for all animals. Results The male rats in test groups including four exposure groups and a recovery group, showed decreased body weight gain. Neutrophilia and lymphocytopenia were determined in females of 3.0 and 6.0 g/kg BW groups and males of 1.5, 3.0 and 6.0 g/kg BW groups by blood routine test (P<0.05). The mean levels of serum ALT and AST significantly increased in males of 3.0 and 6.0 g/kg BW groups (P<0.05). Only the mean level of serum TG in males of 6.0 g/kg BW group decreased (P<0.05). No significant differences of these indexes were shown in female rats of all exposure groups compared to control group. The exposure-related significant increases on the incidences of epidermis and dermis lesion,hepatocellular degeneration and necrosis were observed in test groups compared to the control group. The rats of recovery group were exhibited normal percentages of lymphocytes and neutrophils, levels of ALT,AST and TG.The skin and hepatocellular lesion were improved to some extent, but not recovered exactly. Conclusion In a 4-week repeated-dose toxic study, DCM induce dose-related effects in rats involving skin lesion and liver injury with adverse changes in body weight gain of male rats, part parameters of hematology and serum biochemistry .
Key wordsDichloromethane    Rats    Repeated-dose toxicity    Skin lesion    Liver injury
收稿日期: 2015-07-16      出版日期: 2017-12-07
ZTFLH:  R114  
基金资助:浙江省实验动物科技计划(2013C37027); 浙江省重点科技创新团队资助项目(2011R50021)
通信作者: 孟真,E-mail: zhmeng@cdc.zj.cn   
作者简介: 孟真,博士,副主任技师,主要从事毒理学检验工作
引用本文:   
孟真, 陈苘, 吴惠岭, 夏勇, 傅剑云, 张世鑫, 朱周靓. 二氯甲烷重复剂量经皮染毒对大鼠毒作用的研究[J]. 预防医学, 2016, 28(3): 234-239.
MENG Zhen, CHEN Qing, WU Hui-ling, XIA Yong, FU Jian-yun, ZHANG Shi-xin, ZHU Zhou-jin. A study on the effect of repeated-dose toxicity on rats exposed to dichloromethane by dermal route for 28 days. Preventive Medicine, 2016, 28(3): 234-239.
链接本文:  
http://www.zjyfyxzz.com/CN/      或      http://www.zjyfyxzz.com/CN/Y2016/V28/I3/234
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