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预防医学  2016, Vol. 28 Issue (3): 221-225    
  论著 本期目录 | 过刊浏览 | 高级检索 |
石棉暴露人群血浆中差异microRNA筛选及功能分析
尹先宏, 夏海玲, 蒋兆强, 余珉, 武为, 张敏, 朱丽瑾, 陈钧强, 肖芸, 应士波, 楼建林, 张幸
浙江省医学科学院职业病防治研究所,浙江 杭州 310013
An analysis on plasma microRNA profiling and function among population exposed to asbestos
YIN Xian-hong, XIA Hai-ling, JIANG Zhao-qiang, YU Min, WU Wei, ZHANG Min, ZHU Li-jin, CHEN Jun-qiang, XIAO Yun, YING Shi-bo, LOU Jian-lin, ZHANG Xing
Institute of occupational diseases prevention and control, Zhejiang Academy of Medical Sciences,Hangzhou, Zhejiang, 310013, China
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摘要 目的 探讨石棉暴露对接触人群血浆microRNA(miRNA)表达的影响。方法 选取暴露组(暴露年限>10年)和对照组(未暴露)各3人,用miRNA芯片比较两组对象血浆miRNA表达差异,使用Target Scan、miRanda和PicTar 3种在线软件对差异表达miRNA进行靶基因预测,最后对靶基因进行基因本体论分析和全基因组及代谢途径数据库通路分析。结果 miRNA芯片筛测结果显示,石棉暴露人群血浆中40个miRNA的表达与对照组存在差异(P<0.05),其中有9个miRNA信号值>500。对这些信号值>500的miRNA的靶基因进行预测和通路分析,有5个miRNA检索到相应的靶基因,这些基因主要参与肿瘤相关的信号通路和代谢通路,包括FGFR3等潜在功能靶点。结论 石棉暴露可致接触人群血浆中miRNA发生差异表达,其靶基因主要参与肿瘤相关的信号通路,但其在石棉相关疾病中的作用机制有待进一步研究。
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尹先宏
夏海玲
蒋兆强
余珉
武为
张敏
朱丽瑾
陈钧强
肖芸
应士波
楼建林
张幸
关键词 石棉暴露血浆microRNA靶基因    
AbstractObjective To investigate the effects of asbestos exposure on plasma miRNA expression. Methods Plasma samples were collected from control group and asbestos-exposed group (time of exposure >10 years) and three samples from each group were selected to detect differentially expressed miRNA using LC Sciences miRNA Microarray-Single. The target genes of differential miRNA were predicted by three kinds of online software, Target Scan,miRanda and PicTar.GO term enrichment and KEGG pathways were analyzed. Results The results of microarray indicated that there were 40 differential miRNA expression between exposed and control groups(P<0.05), and the signal value of 9 differential miRNA exceeded 500. After analyzing signal pathways of target genes of 5 miRNA, of which the signal values were over 500, these target genes were found mainly involved in pathways associated with cancer and metabolism, including potential function targets of FAS, TP53 and FGFR3. Conclusion Asbestos exposure can result in differentially expressed miRNA in the plasma from workers occupationally exposed to asbestos and the target genes of these miRNA may play important roles in the pathways of cancer. However, the mechanism of these miRNA in asbestos-related diseases needs to be further studied in the future.
Key wordsAsbestos exposure    Plasma    MicroRNA    Target gene
收稿日期: 2015-08-05      出版日期: 2017-12-07
ZTFLH:  R135  
基金资助:国家自然科学基金(81302464); 国家自然科学基金(81402718); 浙江省重大科技专项重点社会发展项目(2014C03030); 浙江省医药卫生科技计划项目平台骨干人才项目(2014RCA003); 浙江省医药卫生科技计划项目(2015120487)
通信作者: 张幸,E-mail: xingtyou@mail.hz.zj.cn   
作者简介: 尹先宏,硕士,主要从事石棉引起相关疾病的分子生物学机制研究工作
引用本文:   
尹先宏, 夏海玲, 蒋兆强, 余珉, 武为, 张敏, 朱丽瑾, 陈钧强, 肖芸, 应士波, 楼建林, 张幸. 石棉暴露人群血浆中差异microRNA筛选及功能分析[J]. 预防医学, 2016, 28(3): 221-225.
YIN Xian-hong, XIA Hai-ling, JIANG Zhao-qiang, YU Min, WU Wei, ZHANG Min, ZHU Li-jin, CHEN Jun-qiang, XIAO Yun, YING Shi-bo, LOU Jian-lin, ZHANG Xing. An analysis on plasma microRNA profiling and function among population exposed to asbestos. Preventive Medicine, 2016, 28(3): 221-225.
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http://www.zjyfyxzz.com/CN/      或      http://www.zjyfyxzz.com/CN/Y2016/V28/I3/221
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