Hyperlipidemia and intestinal microflora distributions among flying personnel
WANG Dong1, CHEN Jikuai2, CAI Wenjie1, CHEN Yuetao1, JIANG Chunyan3, YU Xiaohong1
1. Department of Rehabilitative Physioltherapy of the Third Sanitarium Area, Air Force Health Care Center Special Services, Hangzhou, Zhejiang 310004, China; 2. Naval Medical University, Shanghai 200433, China; 3. No.903 Hospital of the PLA, Hangzhou, Zhejiang 310013, China
Abstract:Objective To investigate the characteristics of intestinal microflora among flying personnel with hyperlipidemia, so as to provide insights into prevention of hyperlipidemia among flying personnel. Methods Flying personnel diagnosed with hyperlipidemia in a sanatiorium from October 2020 to February 2021 were included in the hyperlipidemia group, while flying personnel with normal blood lipids during the same period served as controls. Subjects' age, family history, physical examinations and blood testing results were collected from both groups. Fecal samples were collected, and intestinal microflora was sequenced followed by bioinformatics analysis. The diversity and abundance of intestinal microflora were compared, and the key bacteria were screened using LEfSe analysis. Results There were 29 subjects in the hyperlipidemia group with a median age (interquartile range) of 34 (12) years and 25 subjects in the control group with a median age (interquartile range) of 30 (12) years, and all subjects were men. There were no significant differences between the two groups in terms of age, flight duration, smoking, family history of metabolic diseases and waist circumference (P>0.05). The Shannon diversity index of intestinal microflora was lower in the hyperlipidemia group than in the control group (Z=4.370, P=0.026), and there was a significant difference in the overall structure of intestinal flora between the two groups, which were clustered into two groups. LEfSe analysis identified Herbaspirillum, Atopobium and Eggerthella as key microorganisms in the hyperlipidemia group, and Agathobacter, Dialister, norank_Eubacterium_coprostanoligenes_group, Alloprevotella and unclassified Bacteroidales as key microorganisms in the control group. Conclusions The species diversity and relative abundance of intestinal microflora are lower in flying personnel with hyperlipidemia than in those with normal blood lipids. Herbaspirillum, Atopobium and Eggerthella may be the key bacteria contributing to hyperlipidemia among flying personnel.
[1] 中国心血管健康与疾病报告2019[J].心肺血管病杂志,2020,39(9):1145-1156. Annual report on cardiovascular health and diseases inChina 2019[J].J Cardiovasc Pulm Dis,2020,39(9):1145-1156. [2] 詹皓. 飞行人员的氧化应激损伤与防护措施研究进展[J].中华航空航天医学杂志,2015,26(2):148-154. ZHAN H.Adbances on oxidative stress injury of aircrew and protective measures[J].Chin J Aerospace Med,2015,26(2):148-154. [3] CORRALES P,VIDAL-PUIG A,MEDINA-GÓMEZ G.PPARs and metabolic disorders associated with challenged adipose tissue plasticity[J/OL].Int J Mol Sci,2018,19(7)[2022-05-23]. https://doi.org/10.3390/ijms19072124. [4] 王星,王军,郑军,等.2015—2020年飞行人员医学停飞疾病谱分析[J].空军医学杂志,2021,37(5):397-417. WANG X,WANG J,ZHENG J,et al.Disease spectrum of military aircrews from 2015 to 2020[J].Med J Air Force,2021,37(5):397-417. [5] FAN Y,PEDERSEN O.Gut microbiota in human metabolic health and disease[J].Nat Rev Microbiol,2021,19(1):55-71. [6] LE CHATELIER E,NIELSEN T,QIN J,et al.Richness of human gut microbiome correlates with metabolic markers[J].Nature,2013,500(7464):541-546. [7] 张泽鹏,郭展鹏,倪钰玥,等.植物乳杆菌JX025073.1对小鼠血脂和肠道菌群的调节作用[J].预防医学,2021,33(9):893-896. ZHANG Z P,GUO Z P,NI Y Y,et al.Effect of Lactobacillus plantarum JX025073.1 on intestinal floraand blood lipid in mice[J].Prev Med,2021,33(9):893-896. [8] 中国成人血脂异常防治指南修订联合委员会.中国成人血脂异常防治指南(2016年修订版)[J].中华健康管理学杂志,2017,11(1):7-28. Joint Committee Issued Chinese Guideline for the Management of Dyslipidemia in Adults.2016 Chinese guideline for the management of dyslipidemia in adults[J].Chin J Health Manag,2017,11(1):7-28. [9] KULECKA M,FRACZEK B,MIKULA T,et al.The composition and richness of the gut microbiota differentiate the top Polish endurance athletes from sedentary controls[J].Gut Microbes,2020,11(5):1374-1384. [10] 周春蕾,杨翔,谢志辉,等.飞行人员冠状动脉狭窄危险因素调查[J].中华航空航天医学杂志,2018,2(增刊1):200-204. ZHOU C L,YANG X,XIE Z H,et al.Investigation of risk factors of coronay aryery stenosis in flying personnel[J].Chin J Aerospacr Med,2018,2(Suppl.1):200-204. [11] 顾东风,翁建平,鲁向锋.中国健康生活方式预防心血管代谢疾病指南[J].中国循环杂志,2020,35(3):209-230. GU D F,WENG J P,LU X F.Chinese guideline on healthy lifestyle to prevent cardiometabolic diseases[J].Chin Circ J,2020,35(3):209-230. [12] LIU C,ZHAO D,MA W,et al.Denitrifying sulfide removal process on high-salinity wastewaters in the presence of Halomonas sp[J].Appl Microbiol Biotechnol,2016,100(3):1421-1426. [13] SEGATA N,IZARD J,WALDRON L,et al.Metagenomic biomarker discovery and explanation[J/OL].Genome Biol,2011,12[2022-05-23].http://genomebiology.com/2011/11/6/R60. [14] 臧凯丽. 微生态制剂调控与脂代谢相关肠道菌群多样性的相关信息分析[D].天津:天津商业大学,2019. ZANG K L.Analysis of infromation related to the regulation of microecological preparations on the diversity of lipid metabolism-related flore[D].Tianjin:Tianjin University of Commerce,2019. [15] DE GOFFAU M C,LUOPAJÄRVI K,KNIP M,et al.Fecal microbiota composition differs between children with β-cell autoimmunity and those without[J].Diabetes,2013,62(4):1238-1244. [16] LAMBETH S M,CARSON T,LOWE J,et al.Composition,diversity and abundance of gut microbiome in prediabetes and type 2 diabetes[J/OL].J Diabetes Obes,2015,2(3)[2022-05-23].https://doi.org/10.15436/2376-0949.15.031. [17] MENNI C,LIN C,CECELJA M,et al.Gut microbial diversity is associated with lower arterial stiffness in women[J].Eur Heart J,2018,39(25):2390-2397. [18] TREMAROLI V,BÄCKHED F.Functional interactions between the gut microbiota and host metabolism[J].Nature,2012,489(7415): 242-249. [19] WEI W,JIANG W,TIAN Z,et al.Fecal g.Streptococcus and g.Eubacterium_coprostanoligenes_group combined with sphingosine to modulate the serum dyslipidemia in high-fat diet mice[J].Clin Nutr,2021,40:4234-4245. [20] FORBES J D,CHEN C Y,KNOX N Cet al.A comparative study of the gut microbiota in immune-mediated inflammatory diseases-does a common dysbiosis exist?[J/OL].Microbiome,2018,6(1)[2022-05-23].https://doi.org/10.1186/s40168-018-0603-4. [21] 熊静芳,傅国胜.高脂血症患者肠道优势菌群与血清脂质水平相关性研究[J].中国微生态学杂志,2013,25(11):1282-1285,1289. XIONG J F,FU G S.Correlations between gut predominant bacteria and serum lipids in patients with hyperlipidemia[J].Chin J Microecol,2013,25(11):1282-1285,1289.