Abstract:Objective To study the effects of Lycium barbarum polysaccharides ( LBP ) on blood indexes and liver tissue morphology in rats with intrahepatic cholestasis. Methods Sprague-Dawley rats were randomly divided into the control group, the model group, and LBP low, medium and high dose group. The rats in the model group and LBP dose groups were given 60 mg/kg alpha-naphthylisothiocyanate ( ANIT ) by gavage every three days of the experiment, and the rats in the control group were given salad oil instead of ANIT. From the third day, the rats in each dose group were given 40, 150 and 600 mg/kg LBP, and the rats in the model group were given distilled water. After four weeks, the blood and urine indexes were measured, and the morphological changes of liver tissue were observed. Results From the third day of the experiment, the activity of rats in the model group and LBP dose groups decreased, and the color of urine changed to dark yellow. There was no abnormality in the group. In the model group, the levels of serum total bilirubin, direct bilirubin, total bile acid ( TBA ), alkaline phosphatase ( ALP ), γ-glutamyltransferase(γ-GGT), cholesterol, alanine aminotransferase ( ALT ), aspartate aminotransferase ( AST ), white blood cell ( WBC ), percentage of granulocyte, urinary bilirubin, urinary bile acid, liver mass and liver to body ratio were higher than those in the control group, while red blood cell and percentage of lymphocyte were lower than those in the control group ( all P<0.05 ). Pathological changes of liver tissue were observed. The levels of serum TBA, ALP, γ-GGT, ALT, AST, WBC and liver to body ratio in LBP high dose group were lower than those in the model group ( all P<0.05 ). The infiltration of inflammatory cells, proliferation and expansion of bile duct, degeneration and necrosis of liver cells were alleviated. Conclusions LBP can improve the blood indexes and pathological changes of liver tissue in rats with intrahepatic cholestasis at the dosage of 600 mg/kg. Inhibition of inflammatory response and reduction of oxidative stress injury may be the mechanism for alleviating cholestatic liver injury.
[1] 中华医学会肝病学分会,中华医学会消化病学分会,中华医学会感染病学分会.胆汁淤积性肝病诊断和治疗共识(2015)[J].胃肠病学,2016,21(1):39-51. [2] YAN J Y,XIE G X,LIANG C G,et al.Herbal medicine Yinchenhaotang protects against α-naphthylisothiocyanate induced cholestasis in rats[J].Sci Rep,2017,7(1):4211-4225. [3] 刘洪涛,杨玉鹏,任炳楠,等.胆汁淤积性肝病治疗药物的临床研究进展[J].化学试剂,2021,43(1):34-40. [4] 冯彦. 枸杞多糖药理作用与临床应用[J].中国现代药物应用,2016,10(6):278-279. [5] 王莉,叶维佳,吴红静,等.黑果枸杞多糖对大鼠肝损伤的保护研究[J].食品工业科技,2020,41(14):287-296. [6] 杜丽娜,杨燕.胆汁淤积动物模型的构建及应用前景[J].临床肝胆病杂志,2019,35(2):445-447. [7] 欧巧群,钱新华,黄笑群,等.ANIT诱导大鼠亚急性肝内胆汁淤积的生化和病理变化[J].胃肠病学和肝病学杂志,2015,24(4):463-468. [8] 夏勇,傅剑云,蔡德雷,等.某栀子复方中药制剂的安全性评价[J].预防医学,2019,31(8):786-794. [9] 卫双连,汪杰,李德民.獐牙菜苦苷对大鼠胆汁淤积性肝损伤肝纤维化的抑制作用[J].世界华人消化杂志,2016,24(23):3482-3487. [10] 葛均波,徐永健,王辰,等.内科学(第9版)[J].北京:人民卫生出版社,2018:401-425. [11] SUN F X,LI X L,XU C J,et al.Effect of compound Yindan decoction on alpha-naphthylisothiocyanate induced acute intrahepatic cholestasis in rats[J].Tradit Chin Med,2019,39(3):315-323. [12] 冯丽娜,张晓雪,黄剑洁,等. 原发性胆汁性胆管炎并发高脂血症的研究现状[J].临床肝胆病杂志,2021,37(1):221-224. [13] 万学红,卢雪峰.诊断学(第9版)[M].北京:人民卫生出版社,2018:363-366. [14] 夏勇,徐彩菊,鹿伟,等.某复方中药制剂引起大鼠肝肾色素沉积的研究[J].预防医学,2020,32(10):1004-1009. [15] 李梅林,刘建飞,邸多隆,等.枸杞多糖防治化学性肝损伤的药理作用研究[J].食品科技,2018,43(9):238-243. [16] 伍智慧,冉喆,张睛睛,等.枸杞多糖对四氯化碳致急性肝损伤小鼠的保护作用[J].宁夏医科大学学报,2021,43(4):364-369. [17] 魏芬芬,王文娟,贺青华,等.枸杞多糖对小鼠酒精性肝损伤的保护作用及机制研究[J].药物评价研究,2019,42(5):852-857. [18] 陈楚颖,代曼云,徐港铭,等.两种胆汁淤积模型肝毒性及胆汁酸代谢调控模式研究[J].中西医结合肝病杂志,2018,28(4):230-233. [19] JIN F,CHENG D,TAO J Y,et al.Anti-inflammatory and anti-oxidative effects of corilagin in a rat model of acute cholestasis[J/OL].BMC Gastroenterol,2013,13:79(2013-03-03)[2021-07-02].https://pubmed.ncbi.nlm.nih.gov/23641818.DOI:10.1186/1471-230X-13-79. [20] 孟姣,吕振宇,孙传鑫,等.枸杞多糖药理作用研究进展[J].时珍国医国药,2018,29(10):2489-2493. [21] POP C,BERCE C,GHIBU S,et al.Effects of Lycium barbarum L.polysaccharides on inflammation and oxidative stress markers in a pressure overload-induced heart failure rat model[J].Molecules,2020,25(3):466-470. [22] TIAN X J,LIANG T S,LIU Y L,et al.Extraction,structural characterization,and biological functions of Lycium barbarum polysaccharides:a review[J].Biomolecules,2019,9(9):389-400.