某复方中药制剂引起大鼠肝肾色素沉积的研究

doi:10.19485/j.cnki.issn2096-5087.2020.10.008

预防医学

2020, Vol. 32

Issue (10): 1004-1009 DOI: 10.19485/j.cnki.issn2096-5087.2020.10.008

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某复方中药制剂引起大鼠肝肾色素沉积的研究

夏勇¹, 徐彩菊¹, 鹿伟¹, 宋燕华¹, 董晓岚², 傅剑云¹, 郑小燕¹

1.浙江省疾病预防控制中心,浙江杭州 310051;
2.浙江省中医院

Study on pigmentation in the livers and kidneys of rats caused by a compound Chinese medicine preparationXIA

Yong^*, XU Caiju, LU Wei, SONG Yanhua, DONG Xiaolan, FU Jianyun, ZHENG Xiaoyan

^*Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China

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摘要目的分析某复方中药制剂引起大鼠肝、肾色素沉积的色素类别及形成原因。方法设低、中、高剂量组及对照组,分别给予1.0、2.0、4.0 g/kgbw某复方中药制剂及蒸馏水,连续灌胃30 d,分析各组SD大鼠的体重、饮食、血液和病理组织学等与色素代谢相关的指标变化。结果实验第1、2周,低、中、高剂量组和对照组大鼠未见异常症状体征,第3周末起,高剂量组大鼠活动减少,尿液颜色浓黄偏绿,粪便颜色变浅。实验期内无大鼠死亡。各组大鼠体重和体重增重差异均有统计学意义（P<0.05）。各组大鼠总食物利用率、直接胆红素、r-谷氨酰转移酶、碱性磷酸酶水平和肝体比差异均有统计学意义（P<0.05）。大体检查发现高剂量组大鼠肝、肾色泽灰暗偏绿;镜下检查发现高剂量组大鼠肝、肾细胞内均可见深色色素颗粒沉积等改变;组织化学染色证明肝、肾内色素沉积为胆色素。结论在4.0 g/kgbw剂量水平下,某复方中药制剂可引起大鼠肝、肾胆色素沉积,胆色素沉积的主要原因是胆汁淤积。

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	夏勇
	徐彩菊
	鹿伟
	宋燕华
	董晓岚
	傅剑云
	郑小燕

关键词 ：中药制剂, 栀子, 色素沉积, 胆色素

Abstract：Objective To explore the cause and type of pigmentation in the livers and kidneys of rats caused by a compound Chinese medicine preparation. Methods The experiment consist of low, medium, and high dose groups and a control group, the Sprague-Dawley rats in these groups were orally given 1.0, 2.0 and 4.0 g/kgbw of a compound Chinese medicine preparation of gardenia and distilled water for 30 days, respectively. The body weight, diet, hematology and histopathology of the rats in each group were observed for changes in pigment metabolism. Results In the first and second weeks of the experiment, the rats in the low, medium, high dose groups and the control group showed no abnormal symptoms or signs. From the third weekend, the urine of the rats in the high dose group turned thick yellow and green, and the stool color became light. During the experiment, no rats died. There were statistically significant differences in body weights and weight gains among these groups ( P<0.05 ) . There were statistically significant differences in total food utilization, direct bilirubin, r-glutamyl transferase, alkaline phosphatase, and liver/body ratios among these groups ( P<0.05 ). Gross examination revealed that the livers and kidneys of rats in the high dose group were dull and green. Microscopic examination revealed changes in dark pigment particles in the livers and kidneys of rats in the high dose group. Histochemical staining confirmed that pigments in the livers and kidneys were bile pigments. Conclusions At a dosage of 4.0 g/kgbw, a compound Chinese medicine preparation of gardenia can lead to bile pigment deposition in the livers and kidneys of rats due to cholestasis.

Key words： traditional Chinese medicine preparation gardenia pigmentation bile pigment

收稿日期: 2019-12-12 修回日期: 2020-02-12

中图分类号:

R758.42

通信作者: 傅剑云,E-mail：jyfu@cdc.zj.cn

作者简介: 夏勇,本科,主任技师,主要从事卫生毒理学工作

引用本文:

夏勇, 徐彩菊, 鹿伟, 宋燕华, 董晓岚, 傅剑云, 郑小燕. 某复方中药制剂引起大鼠肝肾色素沉积的研究[J]. 预防医学, 2020, 32(10): 1004-1009.
Yong, XU Caiju, LU Wei, SONG Yanhua, DONG Xiaolan, FU Jianyun, ZHENG Xiaoyan. Study on pigmentation in the livers and kidneys of rats caused by a compound Chinese medicine preparationXIA. Preventive Medicine, 2020, 32(10): 1004-1009.

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[1] 王华光,赵瑞,刘丽宏.硫酸羟氯喹致皮肤色素沉着1例并文献复习[J].临床药物治疗杂志,2017,15(9):77-79.
[2] 邱桂霞,李文成,黄冬平,等.经支气管镜术诊断儿童特发性肺含铁血黄素沉着症1 例[J].临床肺科杂志,2018,23(11):2130-2131.
[3] 刘彤华. 诊断病理学[M].3版.北京:人民卫生出版社,2015:321-350.
[4] 夏勇,傅剑云,蔡德雷,等.某栀子复方中药制剂的安全性评价[J].预防医学,2019,31(8):786-794.
[5] 王波,杨洪,高双荣,等.栀子对大鼠肝肾毒性的病理学观察[J].中国实验方剂学杂志,2007,13(5):45-48.
[6] 中华人民共和国国家质量监督检验总局.实验动物配合饲料通用质量标准:GB/T 14924.1—2001[S].北京:中国标准出版社,2001.
[7] 傅剑云,陈苘,张世鑫,等.含咖啡因保健食品30天喂养实验受试物处理与评价[J].预防医学,2016,28(7):649-657.
[8] 朱周靓,严峻,郑云燕,等.杜仲叶安全性的毒理学评价[J].预防医学,2017,29(5):443-448.
[9] 叶应抚,王毓三,申子瑜.全国临床检验操作规程[M].北京:人民卫生出版社,2006:283-289.
[10] 曹守勤,张燕飞.锌原卟啉和尿粪卟啉测定在铅中毒中的诊断价值[J].现代医药卫生,2017,33(21):3308-3310.
[11] 杨利峰,周向梅,赵德明,等.大鼠和小鼠病理变化术语及诊断标准的国际规范[M].北京:中国农业出版社,2019:135-136.
[12] 万学红,卢雪峰.诊断学[M]. 9版.北京:人民卫生出版社,2018:38-385.
[13] 欧巧群,钱新华,黄笑群,等.ANIT诱导大鼠亚急性肝内胆汁淤积的生化和病理变化[J].胃肠病学和肝病学杂志,2015,24(4):463-465.
[14] 卢立国,闫卫彬,孙婷婷,等.成人特发性肺含铁血黄素沉着症1例报道及文献分析[J].齐齐哈尔医学院学报,2015,36(2):236-239.
[15] 陈惠萍,胡伟新,黎磊石.色素沉积导致的急性肾小管坏死[J].肾脏病与透析肾移植杂志,2007,16(1):89-92.
[16] 王燕,陈香宇,李娅,等.急性间歇性卟啉病50例临床特征分析[J].中华内科杂志,2019,58(7):520-524.
[17] 李玉林. 病理学[M].8版.北京:人民卫生出版社,2017:17-20.
[18] 熊小丽, 鄢素琪, 秦欢,等. 大黄素预处理对幼龄大鼠肝内胆汁淤积的保护作用[J].中华当代儿科杂志,2016,18(2):165-171.
[19] ESHRAGHI T,EIDI A,MORTAZAVI P,et al.Magnesium protects against bile duct ligation-induced liver injury in male Wistar rats[J]. Magnes Res,2015,28(1):32-45.
[20] TIAN J Z,YI Y,ZHAO Y,et al.Oral chronic toxicity study of geniposide in rats[J].J Ethnopharmacol,2018,213:166-175.

[1]	夏勇, 傅剑云, 蔡德雷, 张世鑫, 徐彩菊, 陈苘, 严峻. 某栀子复方中药制剂的安全性评价[J]. 预防医学, 2019, 31(8): 786-790,794.

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