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预防医学  2019, Vol. 31 Issue (11): 1136-1139    DOI: 10.19485/j.cnki.issn2096-5087.2019.11.014
  疾病控制 本期目录 | 过刊浏览 | 高级检索 |
金山区柯萨奇病毒A组6型基因特征分析
董兆鹏,孙箐爽,臧昊,王唐,蔡光,宋灿磊,李淑华
上海市金山区疾病预防控制中心,上海 201599
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摘要 目的 分析上海市金山区2017—2018年分离的柯萨奇病毒A组6型(CoxA6)VP1基因特征和氨基酸位点突变,为手足口病防控提供依据。方法 对金山区2017—2018年采集并分离到16株CoxA6分离株VP1全长基因进行扩增、测序、序列比对,构建系统发生树,分析核苷酸和氨基酸同源性以及氨基酸位点突变。结果 金山区16株CoxA6分离株均属于E2亚型同一个进化分支,16株间VP1基因核苷酸同源性为92.3%~97.7%,氨基酸同源性为98.4%~100.0%。与金山区16株分离株同源性最高的是2018年山东分离株MK357081/CHN/SD/JN/2018,核苷酸和氨基酸同源性分别为95.7%~99.3%和98.7%~99.7%。13株CoxA6分离株VP1氨基酸序列存在氨基酸位点变异,涉及8个氨基酸变异位点,但在抗原表位重要位点201D、243H、245R、247Y、248M和249R均未检测到抗原漂移。结论 16株CoxA6分离株核苷酸和氨基酸序列高度同源且位于E2亚型同一进化分支;CoxA6 E2亚型可能是金山区2017—2018年流行的主导基因型,金山区CoxA6 VP1存在氨基酸突变位点但未涉及关键的抗原表位。
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董兆鹏
孙箐爽
臧昊
王唐
蔡光
宋灿磊
李淑华
关键词 柯萨奇病毒A组6型;VP1基因;系统发育分析;同源性分析;抗原表位    
          出版日期: 2019-11-01
中图分类号:  R373.23  
基金资助:上海市卫生计生委科研课题(20184Y0023)
通信作者: 李淑华,E-mail:hsli167@163.com   
作者简介: 董兆鹏,硕士,医师,主要从事肠道病毒分子流行病学研究工作
引用本文:   
董兆鹏,孙箐爽,臧昊,王唐,蔡光,宋灿磊,李淑华. 金山区柯萨奇病毒A组6型基因特征分析[J]. 预防医学, 2019, 31(11): 1136-1139.
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http://www.zjyfyxzz.com/CN/Y2019/V31/I11/1136
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