Please wait a minute...
文章检索
预防医学  2018, Vol. 30 Issue (10): 973-977    DOI: 10.19485/j.cnki.issn2096-5087.2018.10.001
  论著 本期目录 | 过刊浏览 | 高级检索 |
六价铬对人B淋巴母细胞细胞周期及相关基因表达的影响
贾君麟,童延,郭心念,方圆,王晶,陆玮,冯玲芳,夏海玲,蒋兆强,余珉,鞠莉,朱丽瑾,应士波,楼建林
浙江省医学科学院职业病防治研究所,浙江 杭州 310000
Effects of hexavalent chromium on cell cycle and expression of related genes in human B lymphoblastoid cells
JIA Jun-lin,TONG Yan,GUO Xin-nian,FANG Yuan,WANG Jing,LU Wei,FENG Ling-fang,XIA Hai-ling,JIANG Zhao-qiang,YU Min,JU Li,ZHU Li-jin,YING Shi-bo,LOU Jian-lin
Institute for Occupational Hazard Prevention,Zhejiang Academy of Medical Sciences,Hangzhou,Zhejiang 310000,China
全文: PDF(603 KB)  
输出: BibTeX | EndNote (RIS)      
摘要 目的 研究六价铬对人B淋巴母细胞的细胞周期及其相关基因表达的影响。方法 用5 μM的重铬酸钾对人B淋巴母细胞进行染毒,24 h后去毒,将细胞置新鲜培养基继续孵育;对照组采用PBS处理。收集24 h、3 d和7 d三个时间点细胞,分别用流式细胞术和实时荧光定量PCR方法检测细胞周期及相关基因mRNA表达。结果 暴露组细胞染毒24 h和去毒后3 d时G0/G1期细胞比例分别为(70.33±2.03)%和(52.97±1.26)%,分别高于对照组的(46.90±2.65)%和(46.87±1.85)%(均P<0.01),去毒后7 d时两组G0/G1期细胞比例差异无统计学意义(P>0.05)。染毒24 h后,支链氨基酸转氨酶1(BCAT1)、细胞周期素依赖激酶抑制因子1A(CDKN1A)及染色质许可和DNA复制因子1(CDT1)三个基因的mRNA表达均较对照组升高(P<0.05),去毒后3 d均低于对照组(P<0.01),去毒后7 d与对照组差异均无统计学意义(P>0.05)。 结论 六价铬可通过调控BCAT1、CDKN1A和CDT1基因的表达影响细胞周期进程。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
贾君麟
童延
郭心念
方圆
王晶
陆玮
冯玲芳
夏海玲
蒋兆强
余珉
鞠莉
朱丽瑾
应士波
楼建林
关键词 六价铬细胞周期支链氨基酶转氨酶1细胞周期素依赖激酶抑制因子1A染色质许可和DNA复制因子1    
AbstractObjective To investigate the influence of hexavalent chromium exposure on cell cycle and the expression levels of cell cycle related genes in human B lymphoblastoid cells. Methods Human B lymphoblastoid cells were treated with 5 μM potassium dichromate for 24 hours and then incubated in fresh medium. The cells in the control group were treated with phosphate buffer saline. The cell cycle and mRNA expression of related genes after 24 hours,3 days and 7 days were detected by flow cytometry and real-time fluorescent quantitative polymerase chain reaction. Results The proportion of cells in G0/G1 phase after 24 hours and 3 days were higher in the exposure group than in the control group[(70.33±2.03)% vs. (46.90±2.65)%;(52.97±1.26)% vs. (46.87±1.85)%;all P<0.01];there was no significant difference in the proportion of cells in G0/G1 phase between the two groups after 7 days(P>0.05). The mRNA expression levels of branched chain amino acid transferase 1 (BCAT1), cyclin-dependent kinases (CDKN1A) and chromatin licensing and DNA replication factor 1(CDT1)in the exposure group were up-regulated in 24 hours and were down-regulated in 3 days after detoxication (all P<0.05);there was no significant difference in mRNA expression levels between the two groups after 7 days(P>0.05). Conclusio Hexavalent chromium exposure could influence the cell cycle by modifying the mRNA expression levels of BCAT1,CDKN1A and CDT1.
Key wordsHexavalent chromium    Cell cycle    BCAT1    CDKN1A    CDT1
          出版日期: 2018-09-26
中图分类号:  R114  
基金资助:国家自然科学基金(81472960、81001242、81502794);浙江省自然科学基金(LY13H260002);浙江省医药卫生科技计划(2015RCA007)
通信作者: 楼建林,E-mail:jianlinlou@163.com   
作者简介: 贾君麟,硕士在读,主要从事重金属健康效应研究工作
引用本文:   
贾君麟, 童延, 郭心念, 方圆, 王晶, 陆玮, 冯玲芳, 夏海玲, 蒋兆强, 余珉, 鞠莉, 朱丽瑾, 应士波, 楼建林. 六价铬对人B淋巴母细胞细胞周期及相关基因表达的影响[J]. 预防医学, 2018, 30(10): 973-977.
JIA Jun-lin, TONG Yan, GUO Xin-nian, FANG Yuan, WANG Jing, LU Wei, FENG Ling-fang, XIA Hai-ling, JIANG Zhao-qiang, YU Min, JU Li, ZHU Li-jin, YING Shi-bo, LOU Jian-lin. Effects of hexavalent chromium on cell cycle and expression of related genes in human B lymphoblastoid cells. Preventive Medicine, 2018, 30(10): 973-977.
链接本文:  
http://www.zjyfyxzz.com/CN/10.19485/j.cnki.issn2096-5087.2018.10.001      或      http://www.zjyfyxzz.com/CN/Y2018/V30/I10/973
[1] NATH K,SINGH D,SHYAM S,et al. Phytotoxic effects of chromium and tannery effluent on growth and metabolism of Phaseolus mungo Roxb[J] . J Environ Biol,2009,30(2):227- 234.
[2] ACKERLEY D F,BARAK Y,LYNCH S V,et al. Effect of chromate stress on Escherichia coli K-12[J] . J Bacteriol,2006, 188(9):3371-3381.
[3] MOHMAND J,EQANI S A,FASOLA M,et al. Human exposure to toxic metals via contaminated dust:Bio-accumulation trends and their potential risk estimation[J] . Chemosphere,2015,132:142-151.
[4] GIBB H J,LEES P S,WANG J,et al. Extended followup of a cohort of chromium production workers[J] . Am J Ind Med,2015,58(8):905-913.
[5] GANAPATHY S,LI P,LAFONTANT J,et al. Chromium IV exposure,via Src/Ras signaling,promotes cell transformation[J] . Mol Carcinog,2017,56(7):1808-1815.
[6] CHAMPERIS T S,KANELLAKIS N,SYMEONIDOU I E,et al. Licensing of DNA replication,cancer,pluripotency and differentiation:an interlinked world[J] . Semin Cell Dev Biol,2014,30:174-180.
[7] PAVEY S,SPOERRI L,HAASS N K,et al. DNA repair and cell cycle checkpoint defects as drivers and therapeutic targets in melanoma[J] . Pigment Cell Melanoma Res,2013,26(6):805-816.
[8] ELIADES P,MILLER D M,MIAO B,et al. A novel multi-CDK inhibitor P1446A-05 restricts melanoma growth and produces synergistic effects in combination with MAPK pathway inhibitors[J] . Cancer Biol Ther,2016,17(7):778-784.
[9] LOU J,WANG Y,YAO C,et al. Role of DNA methylation in cell cycle arrest induced by Cr(VI)in two cell lines[J] . PLoS One,2013,8(8):e71031.
[10] LOU J,JIN L,WU N,et al. DNA damage and oxidative stress in human B lymphoblastoid cells after combined exposure to hexavalent chromium and nickel compounds[J] . Food Chem Toxicol,2013, 55:533-540.
[11] YOBOUE E D,SITIA R,SIMMEN T. Redox crosstalk at endoplasmic reticulum(ER)membrane contact sites(MCS)uses toxic waste to deliver messages[J] . Cell Death Dis,2018,9(3):331.
[12] MADEJCZYK M S,BAER C E,DENNIS W E,et al. Temporal changes in rat liver gene expression after acute cadmium and chromium exposure[J] . PLoS One,2015,10(5):e0127327.
[13] ZHANG Y,XIAO F,LIU X,et al. Cr(VI)induces cytotoxicity in vitro through activation of ROS-mediated endoplasmic reticulum stress and mitochondrial dysfunction via the PI3K/Akt signaling pathway[J] . Toxicol In Vitro,2017,41:232-244.
[14] PARK S,LI C,ZHAO H,et al. Gene 33/Mig6 inhibits hexavalent chromium-induced DNA damage and cell transformation in human lung epithelial cells[J] . Oncotarget,2016,7(8):8916-8930.
[15] XIE H,HOLMES A L,WISE S S,et al. Human skin cells are more sensitive than human lung cells to the cytotoxic and cell cycle arresting impacts of particulate and soluble hexavalent chromium[J] . Biol Trace Elem Res,2015,166(1):49-56.
[16] GORJALA P,CAIRNCROSS J G,GARY R K. p53-dependent up-regulation of CDKN1A and down-regulation of CCNE2 in response to beryllium[J] . Cell Prolif,2016,49(6):698-709.
[17] KARIMIAN A,AHMADI Y,YOUSEFI B. Multiple functions of p21 in cell cycle,apoptosis and transcriptional regulation after DNA damage[J] . DNA Repair (Amst),2016,42:63-71.
[18] CHAYAPONG J,MADHYASTHA H,MADHYASTHA R,et al. Arsenic trioxide induces ROS activity and DNA damage,leading to G0/G1 extension in skin fibroblasts through the ATM-ATR- associated Chk pathway[J] . Environ Sci Pollut Res Int,2017,24(6):5316-5325.
[19] LAZO P A. Reverting p53 activation after recovery of cellular stress to resume with cell cycle progression[J] . Cell Signal,2017,33:49-58.
[20] ZHOU W,FENG X,REN C,et al. Over-expression of BCAT1,a c-Myc target gene,induces cell proliferation,migration and invasion in nasopharyngeal carcinoma[J] . Molecular Cancer,2013,12(1):53.
[21] THEWES V,SIMON R,HLEVNJAK M,et al. The branched- chain amino acid transaminase 1 sustains growth of antiestrogen-resistant and ERα-negative breast cancer[J] . Oncogene,2017, 36(29):4124-4134.
[22] BHATIA B,MALIK A,FERNANDEZ-L A,et al. p27(Kip1),a double-edged sword in Shh-mediated medulloblastoma:Tumor accelerator and suppressor[J] . Cell Cycle,2010,9(21):4307-4314.
[23] COQUERET O. New roles for p21 and p27 cell-cycle inhibitors:a function for each cell compartment[J] . Trends Cell Biol,2003, 13(2):65-70.
[24] ZHANG Y,XING Y,ZHANG L,et al. Regulation of cell cycle progression by forkhead transcription factor FOXO3 through its binding partner DNA replication factor Cdt1[J] . Proc Natl Acad Sci USA,2012,109(15):5717-5722.
[25] YOU Z,ODE K L,SHINDO M,et al. Characterization of conserved arginine residues on Cdt1 that affect licensing activity and interaction with Geminin or Mcm complex[J] . Cell Cycle,2016, 15(9):1213-1226.
[26] TANAKA M,TAKAHARA M,NUKINA K,et al. Mismatch repair proteins recruited to ultraviolet light-damaged sites lead to degradation of licensing factor Cdt1 in the G1 phase[J] . Cell Cycle,2017,16(7):673-684.
[27] MATSON J P,DUMITRU R,CORYELL P,et al. Rapid DNA replication origin licensing protects stem cell pluripotency[J] . Elife,2017,6:e30473.
[1] 吴帆, 冯玲芳, 陈俊斐, 蒋兆强, 龚晓雪, 秦瑶, 楼建林. 六价铬诱发rDNA拷贝数变异对不同细胞系DNA损伤反应的影响[J]. 预防医学, 2023, 35(5): 374-379.
[2] 郭心念, 童延, 贾君麟, 王海明, 冯玲芳, 夏海玲, 蒋兆强, 楼建林. 六价铬对职业人群细胞周期相关基因表达的影响[J]. 预防医学, 2017, 29(7): 670-674.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed