Analysis of semen quality parameters among 155 sperm donors
HUANG Jing1, JIANG Zhaoqiang1, RUAN Zheng1, LI Tao1, SHENG Huiqiang2, LI Jiaping1, LOU Jianlin1,3,4
1. School of Public Health, Hangzhou Medical College, Hangzhou, Zhejiang 310013, China; 2. Human Sperm Bank of Zhejiang Maternal, Child and Reproductive Health Center, Hangzhou, Zhejiang 310013, China; 3. Huzhou Normal University, Huzhou, Zhejiang 313000, China; 4. The First Affiliated Hospital of Huzhou Normal University,Huzhou, Zhejiang 313000, China
Abstract：Objective To analyze the semen quality parameters of sperm donor volunteers, so as to provide insights into male infertility control and related research. Methods A total of 155 sperm donors were recruited from the Human Sperm Bank of Zhejiang Province using the convenience sampling method from January to March 2021. Demographic information were collected through questionnaire surveys. Semen were collected, and parameters including semen volume, sperm concentration, total number of sperm, forward motility rate and total sperm viability were measured. Semen quality was evaluated according to the WHO Laboratory Manual for the Examination and Processing of Human Semen. Results Among 155 sperm donors, 127 were unmarried (81.94%), 97 were unemployed (62.58%), 91 had a bachelor's degree and above (58.71%), and 42 had a body mass index of 25 kg/m2 and above (27.09%). The median age was 24 (interquartile range, 7) years. The median (interquartile range) abstinence time, total number of sperm, semen volume, sperm concentration, forward motility rate and total sperm viability were 4 (1) d, 51 (38) ×106/1 ejaculation, 3.90 (2.20) mL, 204.60 (161.30) ×106/mL, 47% (10%) and 50% (11%), and the unqualified rates were 0%, 0.65%, 1.29%, 4.52%, 9.68% and 18.06%, respectively. The overall unqualified rates of semen quality was 20.65%. Conclusion There were 20.65% of the 155 sperm donors with unqualified semen, and the unqualified rates of forward motility rate and total sperm viability were relatively high.
[1] EISENBERG M L,ESTEVES S C,LAMB D J,et al.Male infertility[J/OL].Nat Rev Dis Primers,2023,9(1)[2024-06-17].https://doi.org/10.1038/s41572-023-00459-w. [2] 杨洋,王树玉.男性不育影响因素的研究进展[J].中国优生与遗传杂志,2011,19(10):131-132. [3] VIRTANEN H E,JORGENSEN N,TOPPARI J.Semen quality in the 21st century[J]. Nat Rev Urol,2017,14(2):120-130. [4] 史美琦,卢莎,吴映瑛,等.高脂饮食对雄性小鼠精子质量及生育能力的影响[J].预防医学,2020,32(5):536-538. [5] NELSON C M,BUNGE R G.Semen analysis:evidence for changing parameters of male fertility potential[J].Fertil Steril,1974,25(6):503-507. [6] LEVINE H,JORGENSEN N,MARTINO-ANDRADE A,et al.Temporal trends in sperm count:a systematic review and meta-regression analysis of samples collected globally in the 20th and 21st centuries[J].Hum Reprod Update,2023,29(2):157-176. [7] LI W N,JIA M M,PENG Y Q,et al.Semen quality pattern and age threshold:a retrospective cross-sectional study of 71 623 infertile men in China,between 2011 and 2017[J].Reprod Biol Endocrinol,2019,17(1):1-8. [8] World Health Organisation.WHO laboratory manual for the examination and processing of human semen[M].Geneva:WHO,2010. [9] 王立宏,何世军,陈卿,等. 73名重庆市大学生精子DNA甲基化水平与精液质量的相关分析[J].陆军军医大学学报,2023,45(9):920-926. [10] 曾云俊. 云南部分地区成年男性精液质量调查与重要影响因素分析[D]. 昆明:昆明医科大学,2021. [11] 葛蕾,谈箐,李航,等.安徽省人类精子库志愿者精液质量与生活方式的关联研究[J].安徽医科大学学报,2024,59(6):952-957. [12] 王文钰,柳松,仕治达,等.1 059名济南市育龄男性体育锻炼情况与精液质量的相关分析[J].陆军军医大学学报,2024,46(10):1164-1171. [13] 李潇,晏斌.北京昌平南部地区1 381例育龄男性精液质量分析[J].中华养生保健,2023,41(20):21-24,29. [14] 崔馨月,王莉,闫聪敏,等.太原市5 876例男性精液质量及其与年龄、禁欲时间的关系[J].中国性科学,2023,32(3):34-37. [15] 赵婉竹,黄云飞,买尔哈巴·阿不力孜,等.西北地区3 400例备孕男性精液质量分析及其影响因素研究[J].中国男科学杂志,2024,38(3):75-81,86. [16] 汤珺壹. 广西某医院成年男性精液质量影响因素分析[D]. 桂林:桂林医学院,2023. [17] 张友旺,潘龙瑞,刘俊,等.线粒体活性对人类精子活动力的影响[J].重庆医科大学学报,2020,45(5):580-584. [18] 赖昱全,孙瑜冲,黄仔冠,等.精子特定形态学与部分精子动力学参数的相关性研究[J].重庆医学,2023,52(7):1000-1003,1008. [19] CALLE-GUISADO V,DE LLERA A H,MARTIN-HIDALGO D,et al.AMP-activated kinase in human spermatozoa:identification,intracellular localization,and key function in the regulation of sperm motility[J].Asian J Androl,2017,19(6):707-714. [20] KUANG W H,ZHANG J,LAN Z,et al.SLC22A14 is a mitochondrial riboflavin transporter required for sperm oxidative phosphorylation and male fertility[J/OL].Cell Rep,2021,35(3)[2024-06-17].https://doi.org.10.1016/j.celrep.2021.109025. [21] CHUNG J J,SHIM S H,EVERLEY R A,et al.Structurally distinct Ca2+ signaling domains of sperm flagella orchestrate tyrosine phosphorylation and motility[J].Cell,2014,157(4):808-822. [22] LIN S Y,KE M,ZHANG Y Q,et al.Structure of a mammalian sperm cation channel complex[J].Nature,2021,595(7869):746-750. [23] 马婧,田慧艳,韩瑞钰,等.空腹血糖、胰岛素抵抗与精液质量的相关性研究[J].预防医学,2019,31(3):274-276,279. [24] 郝睿楠,张倩.男性不育症患者心理健康状况自尊社会支持及婚姻满意度调查[J].临床心身疾病杂志,2022,28(4):52-57. [25] XU S,WU Y R,CHEN Y J,et al.Environmental metal exposure,seminal plasma metabolome and semen quality:evidence from Chinese reproductive-aged men[J/OL].Sci Total Environ,2022,838[2024-06-17].https://doi.org.10.1016/j.scitotenv.2022.155860. [26] ZHANG Y C,SHI W Y,ZHANG M,et al.Exposure to PM2.5,seminal plasma metabolome,and semen quality among Chinese adult men:association and potential mediation analyses[J/OL].J Hazard Mater,2024[2024-06-17].https://doi.org.10.1016/j.jhazmat.2023.132602. [27] YANG P,CHEN D,WANG Y X,et al.Mediation of association between polycyclic aromatic hydrocarbon exposure and semen quality by spermatogenesis-related microRNAs:a pilot study in an infertility clinic[J/OL].J Hazard Mater,2020,384[2024-06-17].https://doi.org.10.1016/j.jhazmat.2019.121431.
