Abstract：Objective To establish a rapid and accurate detecting method for sulfite in food residues.Methods Applying an fluorine ionsalkali-stable liquid to extract ultrasonically.After removing the suspended substances by high-speed centrifugation,we used online dialysis-ion chromatography to determine sulfite. Chromatographic conditionswere set as followsMetrosep A Supp 5-150 / 4.0 anion analysis column,using 3.2 mmol / L sodium carbonate -1.0 mmol / L sodium bicarbonate 5% acetone as the eluent,with 0.70 mL / min flow rate, 20 μL injection volume and a conductivity detector. Results Sulfites (calculated as sulfur dioxide) manifested a good linear relationship when its concentration ranged from 0.50 μg / mL to 50.00 μg / mL,r= 0.999 8.The minimum detection limit was 0.54 mg / kg. The spiked concentrations were 10.0 mg / kg, 100.0 mg / kg, 500.0 mg / kg, 900.0 mg / kg to samples. Relative standard deviation (n=6, %) was 5.04,1.20,0.56,0.98, with the recovery rate between 85.4% -104.4%. Conclusion This method is simple, rapid, with high sensitivity and accuracy of measurement, and which is suitable for the detection of sulfites content in food matrix.
王永根,王剑波,包玲月,朱小琼. 在线渗析-离子色谱法测定食品亚硫酸盐含量研究[J]. 预防医学, 2017, 29(1): 44-48.
WANG Yong-gen,WANG Jian-bo,BAO Ling-yue,ZHU Xiao-qiong. A study on the concentration of sulfites in foods by online dialysis-ion chromatography method. Preventive Medicine, 2017, 29(1): 44-48.
