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预防医学  2018, Vol. 30 Issue (5): 490-493    DOI: 10.19485/j.cnki.issn2096-5087.2018.05.015
  论著 本期目录 | 过刊浏览 | 高级检索 |
HepG2细胞中性红染色法测定化合物急性毒性的研究
徐剑1, 童晓玲2, 岑江杰3, 纪磊3, 林师道3, 朱心强1
1.浙江大学医学院,浙江 杭州 310012;
2.金华市质量技术监督检测院;
3.浙江省化工研究院有限公司
Acute toxicity determination of compounds by HepG2 cell neutral red staining
XU Jian*, TONG Xiao-ling, CEN Jiang-jie, JI Lei, LIN Shi-dao, ZHU Xin-qiang
*School of Medicine,Zhejiang University,Hangzhou,Zhejiang 310058,China
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摘要 目的 探索HepG2细胞中性红染色法检测化合物毒性的可行性,为建立化合物急性毒性快速筛选方法提供依据。方法 采用人肝癌HepG2细胞中性红染色法检测秋水仙碱、环磷酰胺、吡蚜酮等30种化合物的细胞毒性IC50、IC45、 IC40、 IC35和IC30值。采用线性回归模型拟合ICx值对大鼠经口半数致死剂量(LD50)的回归方程,利用回归方程预测LD50值。采用LD50实际值和预测值对化合物毒性进行分级,比较两种方法的一致性。结果 lg(IC50)、lg(IC45)、 lg(IC40)、 lg(IC35)、lg(IC30)与lg(LD50)均有相关性(P<0.01),相关系数(r)为0.779~0.815;lg(IC50)与lg(LD50)的相关性最高,lg(LD50)=0.887×lg(IC50)+0.414。LD50实际值将30种化合物划分为高毒、中等毒和低毒各10种,LD50预测值划分出高毒12种、中等毒10种、低毒8种;两种方法的划分结果差异无统计学意义(P>0.05),一致率为86.7%。结论 人肝癌HepG2细胞中性红染色法可作为快速检测化合物急性毒性的方法。
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徐剑
童晓玲
岑江杰
纪磊
林师道
朱心强
关键词 急性毒性HepG2细胞中性红染色法    
AbstractObjective To explore the feasibility of detecting compound toxicity in HepG2 cell neutral red staining method and to provide the basis for the establishment of a rapid screening method. Methods The cytotoxic IC50,IC45,IC40,IC35 and IC30 values of 30 kinds of compounds,including colchicine,cyclophosphamide pipion and so on,were detected by the neutral red staining method of human liver cancer HepG2 cells. The regression equation of the ICx value was used to predict the LD50 value of the rat via the linear regression method,and the LD50 was predicted by the regression equation with the highest correlation. The toxicity of the compound was graded by the actual value and predicted value of LD50,and the consistency of the two methods was compared. Results The correlation between lg(IC50),lg(IC45),lg(IC40),lg (IC35),lg(IC30)and lg(LD50)was statistically significant(P<0.01),and correlation coefficient(r)was 0.779-0.815. The correlation between lg(IC50)and lg(LD50) is the highest,lg(LD50)=0.887×lg(IC50) + 0.414. The actual value of LD50 divided 30 compounds into 10 kinds of high toxicity,medium toxicity and low toxicity,and the predicted value of LD50 was divided into 12 types of high toxicity,10 medium toxicity and 8 low toxicity. There was no significant difference between the two methods(P>0.05),and the concordant rate was 86.7%. Conclusion The cell neutral red staining method can be used as a method for rapid detection of acute toxicity.
Key wordsAcute toxicity    HepG2 cells    Neutral red staining
收稿日期: 2018-01-02      修回日期: 2018-02-09     
中图分类号:  R99  
通信作者: 朱心强,E-mail:zhuxq@zju.edu.cn   
作者简介: 徐剑,本科,助理研究员,主要从事化学品、农药毒理检测工作
引用本文:   
徐剑, 童晓玲, 岑江杰, 纪磊, 林师道, 朱心强. HepG2细胞中性红染色法测定化合物急性毒性的研究[J]. 预防医学, 2018, 30(5): 490-493.
XU Jian, TONG Xiao-ling, CEN Jiang-jie, JI Lei, LIN Shi-dao, ZHU Xin-qiang. Acute toxicity determination of compounds by HepG2 cell neutral red staining. Preventive Medicine, 2018, 30(5): 490-493.
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http://www.zjyfyxzz.com/CN/10.19485/j.cnki.issn2096-5087.2018.05.015      或      http://www.zjyfyxzz.com/CN/Y2018/V30/I5/490
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