Abstract：ObjectiveTo explore the value of prenatal diagnosis of fetal ABO blood groups in the prevention of ABO-HDN, and to provide evidence for prevention of ABO-HDN.MethodsA total of 3 777 samples were collected from the pregnant women whose ABO blood group is O, and we detected the ABO blood group by serological method to detect the titer of IgG anti-A and anti-B in the maternal blood.ResultsAmong the 3 777 samples collected from the pregnant women whose ABO blood group is O , the titer of IgG anti-A to anti-B was 1to1 024 in 27 samples(0.7%), 1∶512 in 97 samples(2.6%), 1∶256 in 163 samples(4.3%), 1∶128 in 285 samples(7.5%) and 1:64 in 603 samples(16%).We followed the pregnancy and newborn outcome of 769 case whose antibody titer of 1∶64 or more , and compared the fetal ABO blood group with results of the titer of IgG anti-A and/or anti-B. A total of 641 patients (83.3%) was corresponding resistance against A or B, and 128 patients (16.6%) was not corresponding resistance against A or B. The higher the antibody titer, the higher incidence of neonatal ABO hemolytic disease occurred. We extracted the fetal free DNA of peripheral blood plasma in 30 pregnant women, and the genotypes of fetal ABO blood group were detected by the polymerase chain reaction-sequence specific primer (PCR-SSP), and all the experiment presented success. ConclusionThe titer of IgG anti-A to anti-B could be used to prevent the occurrence of hemolytic disease of newborn. Considering the interference factors, the fetal free DNA in the maternal circulation could be used to prenatally detect fetal ABO blood groups.
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