Abstract：Objective To establish an isotope dilution gas chromatography-mass spectrometry（GC-MS） for the determination of chloropropanol esters in fried foods. Methods A total of 88 fried food samples were collected from supermarket,breakfast shop and street breakfast,stalls,the fried food sample with no chloropropanols esters detected was used as the blank sample. Samples were extracted using a solvent extraction method,followed by ester-bond cleavage reaction with sodium methylate-methanol and purification by diatomite solid-supported liquid-liquid extraction column. The derivatives in purified solution was detected by GC-MS after being derivatived with heptafluoro butyrylimidazole. The concentration of chloropropanols esters was quantified by using deuterium isotopes as internal standards. The accuracy of the method for evaluating recovery rate of blank samples was adopted,and the relative standard deviation （RSD） of the recovery rate represents the precision of the method. Results The 3-MCPD ester and 2-MCPD ester had good linear relationship in the concentration range of 25-1 000 g/L（r>0.9995）. The detection limits of 3-MCPD ester and 2-MCPD ester were 20 μg/kg. The recovery rate of fat extracts from blank samples at 25,50,100,and 200 μg/kg levels ranged from 89.7% to 103.7%,and RSD<8.4%. The detection rates of 3-MCPD ester and 2-MCPD ester in 88 samples were 81.82% and 70.45% respectively,and the content ranges from not-detected （ND） to 1.65 mg/kg and to 0.93 mg/kg respectively. Conclusion The method is simple,accurate and reliable. It is suitable for the determination of chloropropanol esters in fried foods. There is a certain degree of contamination of chloropropanol esters in fried foods,and this comtamination is quite common.
王立媛, 吴平谷, 应英, 王天娇, 胡争艳, 沈向红, 俞莎. 油炸食品中氯丙醇酯固相萃取GC-MS测定研究[J]. 预防医学, 2017, 29(9): 878-882.
WANG Li-yuan, WU Ping-gu, YING Ying, WANG Tian-jiao, HU Zheng-yan, SHEN Xiang-hong, YU Sha. A study on the determination of chloropropanols esters in fried foods by solid-phase extracting method and GC-MS. Preventive Medicine, 2017, 29(9): 878-882.
JECFA. 3-Chloro-1,2-propane-diol （addendum）. in：safety evaluation of certain food additives and contaminants,prepared by the sixty-seventh meeting of the Joint FAO/WHO Expert Committee on Food Additives（JECFA）,WHO food additives series 58,2007,pp. 239-266.
[2]
HWANG M,YOON E,KIM J,et al. Toxicity value for 3-monochloropropane-1,2-diol using a benchmark dose methodology[J]. Regul Toxicol Pharmacol,2009,53（2）：102-106.
[3]
HAMLET C G,ASUNCION L,VELÍŠEK J,et al. Formation and occurrence of esters of 3-chloropropane-1,2-diol （3-CPD）in foods：what we know and what we assume[J]. European Journal of Lipid Science and Technology,2011,113（3）：279-303.
[4]
ZELINKOVÁ Z,NOVOTNY O,SCHUREK J,et al. Occurrence of 3-MCPD fatty acid esters in human breast milk[J]. Food Addit Contam Part A Chem Anal Control Expo Risk Assess,2008,25（6）：669-676.
[5]
CHUNG H Y,CHUNG S W,CHAN B T,et al. Dietary exposure of Hong Kong adults to fatty acid esters of 3-monochloropropane-1,2-diol[J]. Food Addit Contam Part A Chem Anal Control Expo Risk Assess,2013,30（9）：1508-1512.
[6]
ZELINKOVÁ Z,DOLEZAL M,VELÍŠEK J. Occurrence of 3-chloropropane-1,2-diol fatty acid esters in infant and baby foods[J]. European Food Research and Technology,2008, 228（4）：571-578.
[7]
ZELINKOVÁ Z,SVEJKOVSKÁ B,VELÍŠEK J,et al. Fatty acid esters of 3-chloropropane-1,2-diol in edible oils[J]. Food Addit Contam,2006,23（12）：1290-1298.
[8]
SVEJKOVSKA B,NOVOTNY O,DIVINOVA V,et al. Esters of 3-chloropropane-1,2-diol in foodstuffs[J]. Czech Journal of Food Sciences,2004,22（5）：190-196.
[9]
ZHOU H,JIN Q,WANG X,et al. Direct measurement of 3-chloropropane-1,2-diol fatty acid esters in oils and fats by HPLC method[J]. Food Control,2014,36（1）：111-118.
[10]
HORI K,KORIYAMA N,OMORI H,et al. Simultaneous determination of 3-MCPD fatty acid esters and glycidol fatty acid esters in edible oils using liquid chromatography time-of-flight mass spectrometry[J]. LWT - Food Science and Technology,2012,48（2）：204-208.
[11]
DIVINOVA V,SVEJKOVSKA B,DOLEZAL M,et al.Determination of free and bound 3-chloropropane-1,2-diol by gas chromatography with mass spectrometric detection using deuterated 3-chloropropane-1,2-diol as internal standard. Czech Journal of Food Sciences,2004,22（5）：182-189.
[12]
DGF Standard Method C III 18b（2009）：Determination of ester-bound 3-chloropropane-1,2-diol（3-MCPD esters）and 3-MCPD forming substances in fats and oils by means of GC-MS[EB/OL]. http：//www.pdfio.com/k- 334356.html.
[13]
BORNSCHEUER U T,HESSELER M. Enzymatic Removal of 3-Monochloropropanediol （3-MCPD）and its Esters from Oils[J]. European Journal of Lipid Science and Technology,2010,112（5）：552-556.
[14]
RAZAK R A,KUNTOM A,SIEW W L,et al. Detection and monitoring of 3-monochloropropane-1,2-diol （3-MCPD） esters in cooking oils[J]. Food Control,2012,25（1）：355- 360.
[15]
LIU Q,HAN F,XIE K,et al. Simultaneous determination of total fatty acid esters of chloropropanols in edible oils by gas chromatography-mass spectrometry with solid-supported liquid-liquid extraction[J]. J Chromatogr A,2013,1314：208-215.
[16]
MIYAZAKI K,KOYAMA K,SASAKO H,et al. Indirect Method for Simultaneous Determinations of 3-Chloro-1, 2-Propanediol Fatty Acid Esters and Glycidyl Fatty Acid Esters[J]. Journal of the American Oil Chemists' Society,2012,89（8）：1403-1407.
