Abstract：Benzo [a] pyrene ( B [a] P ) is a well-recognized environmental pollutant. Exposure to B[a]P elicits many adverse biological effects, including tumorigenesis, immunosuppression, teratogenicity, and hormonal effects. In addition to B [a] P exposure-induced genetic damages, a growing number of studies demonstrate that epigenetic changes play an important role in chemically induced carcinogenesis. In order to provide better understanding of epigenetic changes of B [a] P and their potential association with genotoxic endpoints, this review summarizes the advances in the applications of functional genomics in the research of B [a] P toxicity, including functional genomics techniques, regulation of human genome expression, DNA sequence variability, model organisms research, and bioinformatics studies, so as to provide insights into the management of B [a] P exposure-induced health injuries and use of genomics techniques to unravel the mechanisms underlying the toxicity of other environmental pollutants.
曹彬, 宋燕华. 功能基因组学在苯并[a]芘毒性研究中的应用进展[J]. 预防医学, 2022, 34(2): 151-155,160.
CAO Bin, SONG Yanhua. Applications of functional genomics in research of benzo[a]pyrenetoxicity：a review. Preventive Medicine, 2022, 34(2): 151-155,160.
[1] VERMA N,PINK M,RETTENMEIER A W,et al.Review on proteomic analyses of benzo[a]pyrene toxicity[J].Proteomics,2012,12(11):1731-1755. [2] LIU Z,HUANG R,ROBERTS R,et al.Toxicogenomics:a 2020 vision[J].Trends Pharmacol Sci,2019,40(2):92-103. [3] SUNDBERG C D,HANKINSON O.A CRISPR/Cas9 whole-genome screen identifies genes required for aryl hydrocarbon receptor-dependent induction of functional CYP1A1[J].Toxicol Sci,2019,170(2):310-319. [4] LIAMIN M,LE MENTEC H,EVRARD B,et al.Genome-wide transcriptional and functional analysis of human T lymphocytes treated with benzo[a]pyrene[J].Int J Mol Sci,2018,19(11):1-18. [5] HU J C,LI W T,ADEBALI O,et al.Genome-wide mapping of nucleotide excision repair with XR-seq[J].Nat Protic,2019,14(1):248-282. [6] ZUO J,BREWER D S,ARLT V M,et al.Benzo pyrene-induced DNA adducts and gene expression profiles in target and non-target organs for carcinogenesis in mice[J/OL].BMC Genomics,2014,15(1)[2021-11-12].https://doi.org/10.1186/1471-2164-15-880. [7] ZHANG C M,SUN Z X,WANG Z L,et al.Abnormal methylation of spermatozoa induced by benzo(a)pyrene in rats[J].Hum Exp Toxicol,2019,38(7):846-856. [8] PAKZAD TOOCHAEI S,GHASEMPOURI S M,RIYAHI BAKHTIARI A,et al.Global DNA methylation changes in rock pigeon (Columba livia)as a sentinel species due to polycyclic aromatic hydrocarbons exposure in Tehran(Iran)as a megacity[J].Environ Sci Pollut Res Int,2019,26(25):26090-26101. [9] HERBSTMAN J B,TANG D L,ZHU D G,et al.Prenatal exposure to polycyclic aromatic hydrocarbons,benzo[a]pyrene-DNA adducts,and genomic DNA methylation in cord blood[J].Environ Health Perspect,2012,120(5):733-738. [10] SADIKOVIC B,RODENHISER D I.Benzopyrene exposure disrupts DNA methylation and growth dynamics in breast cancer cells[J].Toxicol Appl Pharmacol,2006,216(3):458-468. [11] TOMMASI S,ZHENG A,YOON J I,et al.Epigenetic targeting of the nanog pathway and signaling networks during chemical carcinogenesis[J].Carcinogenesis,2014,35(8):1726-1736. [12] FU I,CAI Y Q,GEACINTOV N E,et al.Nucleosome histone tail conformation and dynamics:impacts of lysine acetylation and a nearby minor groove benzo[a]pyrene-derived lesion[J].Biochemistry,2017,56(14):1963-1973. [13] SADIKOVIC B,ANDREWS J,CARTER D,et al.Genome-wide H3K9 histone acetylation profiles are altered in benzopyrene-treated MCF7 breast cancer cells[J].J Biol Chem,2008,283(7):4051-4060. [14] ZAPLETAL O,TYLICHOVÁ Z,NEA J,et al.Butyrate alters expression of cytochrome P450 1A1 and metabolism of benzo[a]pyrene via its histone deacetylase activity in colon epithelial cell models[J].Arch Toxicol,2017,91(5):2135-2150. [15] LIANG J R,ZHU H Y,LI C Z,et al.Neonatal exposure to benzo[a]pyrene decreases the levels of serum testosterone and histone H3K14 acetylation of the StAR promoter in the testes of SD rats[J].Toxicology,2012,302(2/3):285-291. [16] BARKLEY L R,SANTOCANALE C.MicroRNA-29a regulates the benzo[a]pyrene dihydrodiol epoxide-induced DNA damage response through Cdc7 kinase in lung cancer cells[J/OL].Oncogenesis,2013,2(7)[2021-11-12].https://doi.org/10.1038/oncsis.2013.20. [17] CHOI Y M,AN S,LEE E M,et al.CYP1A1 is a target of miR-892a-mediated post-transcriptional repression[J].Int J Oncol,2012,41(1):331-336. [18] GORDON M W,YAN F,ZHONG X M,et al.Regulation of p53-targeting microRNAs by polycyclic aromatic hydrocarbons:implications in the etiology of multiple myeloma[J].Mol Carcinogen,2015,54(10):1060-1069. [19] HAN Z Y,ZHANG Y,XU Y,et al.Cell cycle changes mediated by the p53/miR-34c axis are involved in the malignant transformation of human bronchial epithelial cells by benzo[a]pyrene[J].Toxicol Lett,2014,225(2):275-284. [20] FILIPPOV S V,YARUSHKIN A A,YAKOVLEVA A K,et al.Effect of benzo(a)pyrene on the expression of AhR-regulated microRNA in female and male rat lungs[J].Biomed Khim,2020,66(3):224-232. [21] CHANYSHEV M D,KOVAL O A,NUSHTAEVA A A,et al.Effect of benzo[a]pyrene on the expression of miR-126,miR-190a and their target genes egfl7,tp53inp1 and phlpp1 in primary endometrial cells[J/OL].J Biochem Mol Toxicol,2019,33(6)[2021-11-12].https://onlinelibrary.wiley.com/doi/10.1002/jbt.22314. [22] GAO L,MAI A,LI X,et al.LncRNA-DQ786227-mediated cell malignant transformation induced by benzo(a)pyrene[J].Toxicol Lett,2013,223(2):205-210. [23] YANG Q,ZHANG S,LIU H,et al.Oncogenic role of long noncoding RNA AF118081 in anti-benzo[a]pyrene-trans-7,8-dihydrodiol-9,10-epoxide-transformed 16HBE cells[J].Toxicol Lett,2014,229(3):430-439. [24] ZHOU S F,LIU J P,CHOWBAY B.Polymorphism of human cytochrome P450 enzymes and its clinical impact[J].Drug Metab Rev,2009,41(2):89-295. [25] BEAL M A,GAGNE R,WILLIAMS A,et al.Characterizing benzo[a]pyrene-induced lacZ mutation spectrum in transgenic mice using next-generation sequencing[J/OL].BMC Genom,2015,16(812)[2021-11-12].https://doi.org/10.1186/s12864-015-2004-4. [26] YU X J,YANG M J,ZHOU B,et al.Characterization of somatic mutations in air pollution-related lung cancer[J].EBioMedicine,2015,2(6):583-590. [27] IZHAR L,ZIV O,COHEN I S,et al.Genomic assay reveals tolerance of DNA damage by both translesion DNA synthesis and homology-dependent repair in mammalian cells[J].Proc Natl Acad Sci U S A,2013,110(16):E1462-E1469. [28] KIM B M,KANG S,KIM R O,et al.De novo transcriptome assembly of brackish water flea Diaphanosoma celebensis based on short-term cadmium and benzo[a]pyrene exposure experiments[J/OL].Herditas,2018,155[2021-11-12].https://doi.org/10.1186/s41065-018-0075-3. [29] JUNG J H,MOON Y S,KIM B M,et al.Comparative analysis of distinctive transcriptome profiles with biochemical evidence in bisphenol S - and benzo[a]pyrene-exposed liver tissues of the olive flounder Paralichthys olivaceus[J].PLoS One,2018,13(5):1-25. [30] JEBALI J,CHICANO-GÁLVEZ E,BANNI M,et al.Biochemical responses in seabream(Sparus aurata)caged in-field or exposed to benzo(a)pyrene and paraquat characterization of glutathione S-transferases[J].Ecotoxicol Environ Saf,2013,88:169-177. [31] CARVALHO R N,LETTIERI T.Proteomic analysis of the marine diatom thalassiosira pseudonana upon exposure to benzo(a)pyrene[J].BMC Genomics,2011,12:1-11. [32] MOFFAT I,CHEPELEV N,LABIB S,et al.Comparison of toxicogenomics and traditional approaches to inform mode of action and points of departure in human health risk assessment of benzo[a]pyrene in drinking water[J].Crit Rev Toxicol,2015,45(1):1-43. [33] YIN J F,WANG Z X,SONG M Y,et al.Plastic antibody for DNA damage:fluorescent imaging of BPDE-dG adducts in genomic DNA[J].Analyst,2013,138(17):4958-4966. [34] ZHANG Q L,GUO J,DENG X Y,et al.Comparative transcriptomic analysis provides insights into the response to the benzo(a)pyrene stress in aquatic firefly(Luciola leii)[J].Sci Total Environ,2019,661:226-234. [35] ZHANG Q L,JIANG Y H,ONG Z X,et al.Exposure to benzo[a]pyrene triggers distinct patterns of microRNA transcriptional profiles in aquatic firefly Aquatica wuhana(Coleoptera: Lampyridae)[J/OL].J Hazard Mater,2021,401[2021-11-12].https://doi.org/10.1016/j.jhazmat.2020.123409.
