Abstract：Objective To establish a method for the determination of furans in tea by headspace-gas chromatographic mass spectrometry. Methods The 20% sodium chloride solution and isotope internal standards were added to the crushed and weighed tea sample. Furan, 2-methylfuran, 3-methylfuran, 2,5-dimethylfuran were separated by HP-PLOT Q capillary column and then determined by gas chromatography mass spectrometry with electron impact ionization mode. Results In the range of 5-400 ng, good linear relationships were observed in the four furan compounds, with the correlation coefficients ranging from 0.999 2 to 0.999 6. The detection limits ranged from 0.2 to 1.9 μg/kg, the quantification limit ranged from 0.4 to 3.1 μg/kg. The recovery rates of furans ranged from 95.4% to 128.2% when spiked at 5.0, 20.0 and 100.0 μg/kg, and the relative standard deviations ranged from 0.8% to 11.3%. Eighty-one tea samples were determined, the concentration of four furan compounds was highest in black tea, followed by dark tea, oolong tea, green tea and scented tea. Conclusion Headspace-gas chromatography-mass spectrometry can reduce the matrix interference of tea, and meet the requirements in the linear range, recovery and precision, which is suitable for simultaneous determination of four furan compounds in tea.
[1] US Food and Drug Administration.Exploratory data on furan in food[EB/OL].[2020-10-10].https://www.fda.gov/food/chemicals/exploratory-data-furan-food. [2] National Toxicology Program.Toxicology and carcinogenesis studies of furan in F344 rats and B6C3F1 mice[J].Natl Toxicol Program Tech Rep Ser,1993,402:1-286. [3] BI K H,ZHANG L,QIAO X G,et al.Tea Polyphenols as inhibitors of furan formed in the maillard model system and canned coffee model[J].J Food Sci,2017,82(5):1271-1277. [4] 冯新昌,李拥军.食品中呋喃的检测方法研究进展[C]// 甘青宁色谱协作中心,甘肃省化学会色谱委员会.西北地区第七届色谱学术报告会甘肃省第十二届色谱年会论文集.兰州:甘青宁色谱协作中心,甘肃省化学会色谱委员会,2012:26-28. [5] 刘晓毅,石维妮,赵玉琪,等.顶空固相微萃取法测定食品中的呋喃[J].食品科技,2010,35(9):297-300. [6] 刘平,薛颖,金庆中,等.顶空气相色谱-质谱法测定婴幼儿食品中的呋喃[J].色谱,2008,26(1):35-38. [7] 何碧英,孙健,柳洁,等.顶空气相色谱-质谱法测定食品中呋喃[J].中国卫生检验杂志,2009,19(12):2790-2792. [8] HU G,ZHU Y,HERNANDEZ M,et al.An efficient method for the simultaneous determination of furan,2-methylfuran and 2-pentylfuran in fruit juices by headspace solid phase microextraction and gas chromatography-flame ionisation detector[J].Food Chem,2016,192(192):9-14. [9] ALTAKI M S,SANTOS F J,GALCERAN M T.Automated headspace solid-phase microextraction versus headspace for the analysis of furan in foods by gas chromatography-mass spectrometry[J].Talanta,2009,78(4/5):1315-1320. [10] MARIKA J,TALVIKKI J,EILA J,et al.Furan in the baby-food samples purchased from the Finnish markets-Determination with SPME-GC-MS[J].Food Chem,2009,117(3):522-528. [11] CHAICHI M,MOHAMMADI A,HASHEMI M.Optimization and application of headspace liquid-phase microextraction coupled with gas chromatography-mass spectrometry for determination of furanic compounds in coffee using response surface methodology[J].Microchem J,2013,108(2):46-52. [12] 柴晓玲,郝雅茹,李书国.电化学分析法快速测定食品中的呋喃[J].食品科学,2019,40(16):256-260. [13] WU P G,ZHANG L Q,HU Z Y,et al.Contamination of 15+1 European Union polycyclic aromatic hydrocarbons in various types of tea and their infusions purchased on Hangzhou city market in China[J].Food Addit Contam,37(10):1621-1632.
