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预防医学  2019, Vol. 31 Issue (12): 1225-1227,1232    DOI: 10.19485/j.cnki.issn2096-5087.2019.12.008
  论著 本期目录 | 过刊浏览 | 高级检索 |
昆明种小鼠肺肿瘤模型的建立
金伟1, 雷林2
1.四川省疾病预防控制中心,四川 成都 610041;
2.四川省自然资源科学研究所
The establishment of Kunming mouse models of lung tumor
JIN Wei*, LEI Lin
*Sichuan Provincial Center for Disease Control and Prevention,Chengdu,Sichuan 610041,China
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摘要 目的 采用尿烷和二丁基羟基甲苯建立昆明种小鼠肺肿瘤短期诱发模型。方法 200只SPF级昆明种小鼠,雌雄各半,随机纳入低、中、高3个剂量组和对照组。低、中、高剂量组小鼠分别一次性腹腔注射50、100、200 mg/kgbw尿烷,1周后每天喂饲含0.75%二丁基羟基甲苯的软饲料,连续2周;对照组注射蒸馏水,每天喂饲普通软饲料。第15周末处死小鼠并取出肺脏,计数各叶肺表面肿瘤数并固定;将肿瘤逐个取材,余下肺组织切成1~2 mm厚的组织块,行常规石蜡切片、HE染色并在光镜下观察。结果 低、中、高剂量组和对照组小鼠肿瘤发生率分别为14.00%、24.00%、38.00%和2.00%,发生肿瘤数分别为8、22、47和1个,肿瘤恶化率分别为12.50%、18.18%、27.66%和0。中、高剂量组小鼠肺肿瘤发生率、肿瘤数均大于对照组(P<0.05);各剂量组小鼠肺肿瘤发生率、肿瘤数和肿瘤恶化率均随尿烷剂量增加而增加,呈剂量-反应关系(P<0.05)。结论 昆明种小鼠肺肿瘤短期诱发模型建模成功,剂量-反应关系明显,成瘤率较高。
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金伟
雷林
关键词 肺肿瘤尿烷二丁基羟基甲苯小鼠模型    
AbstractObjective To establish Kunming mouse models of lung tumor rapidly induced by urethane and butylated hydroxytoluene(BHT). Methods Two hundred Kunming mice of SPF grade were randomly assigned to one control group and three experimental groups. The mice in the experimental groups were intraperitoneally injected with urethane at the dosage of 50,100 and 200 mg/kg(low,medium and high dose),after one week,they were fed with fodder containing 0.75% BHT every day for two weeks;while the mice in the control group were injected with distilled water and were fed with normal fodder without BHT. At the 15th week,the lungs of all the mice were removed,tumors were counted and collected. The remaining lung tissue was cut into blocks of 1-2 mm thick,followed by paraffin section,HE staining,and was observed under light microscope. Results The incidence rates of lung tumors in Kunming mice of the low-,meidium-,high-dose group and the control group were 14.00%,24.00%,38.00% and 2.00%;the number of tumors were 1,8,22 47;the proportion of malignant tumors were 12.50%,18.18%,27.66% and 0. The incidence rate and the number of tumors in medium- and high-dose group were significantly higher than those in the control group(P<0.05). The incidence rate,the number of tumors and the proportion of malignant tumors increased with the dosage of urethane,which indicated a dose-response relationship(P<0.05). Conclusion The Kunming mouse models of lung tumor rapidly induced by urethane and BHT were established successfully,with significant dose-response relationship and tumorigenesis.
Key wordsLung tumor    Urethane    Butylated hydroxytoluene    Mouse model
收稿日期: 2019-06-06      修回日期: 2019-09-19     
中图分类号:  R734.2  
通信作者: 金伟,E-mail:qgmw6970@163.com   
作者简介: 金伟,硕士,副主任医师,主要从事卫生毒理工作
引用本文:   
金伟, 雷林. 昆明种小鼠肺肿瘤模型的建立[J]. 预防医学, 2019, 31(12): 1225-1227,1232.
JIN Wei, LEI Lin. The establishment of Kunming mouse models of lung tumor. Preventive Medicine, 2019, 31(12): 1225-1227,1232.
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http://www.zjyfyxzz.com/CN/10.19485/j.cnki.issn2096-5087.2019.12.008      或      http://www.zjyfyxzz.com/CN/Y2019/V31/I12/1225
[1] 陈万青,张思维,邹小农.中国肺癌发病死亡的估计和流行趋势研究[J].中国肺癌杂志,2010,13(5):488-493.
[2] 张晓,杨琼英,林国桢,等. 大气污染与居民肺癌发病及死亡灰色关联分析[J]. 中国公共卫生,2014,30(2):165-170.
[3] DEVESA S S,BRAY F,VIZCAINO P,et al.International lung cancer trends by histologic type:male:female differences diminishing and adenocarcinoma rates rising[J].Int J Cancer,2005,17(2):294-299.
[4] FONTHAM E T,CORREA P,REYNOLDS P,et al.Environmental tobacco smoke and lung cancer in nonsmoking women:a multicenter study[J]. JAMA,1994,271(22):1752-1759.
[5] VIKIS H G,RYMASZEWSKI A L,TICHETAAR J W.Mouse models of chemically-induced lung carcinogenesis[J].Front Biosci,2013,E5(3):939-946.
[6] WILIAMS G M,WEISBURGER J H.Chemical carcinogenesis[M]// Casarett and Doull's Toxicology. New York:Pergamon Press,1991:127-200.
[7] 余琛琳,崔淑芳. 肺癌动物模型的制备与应用[J]. 中国实验动物学报,2008,16(6):470-474.
[8] KIM J,KIM Y.Animal models in carotenoids research and lung cancer prevention[J]. Trans Oncology,2011,4(5):271-281.
[9] 顾其华,胡成平,夏莹,等. 3,4-苯并芘肺内注射构建大鼠肺肿瘤模型的实验研究[J]. 中国肺癌杂志,2007,10(3):172-175.
[10] KIRSH I X,GRIMM J,GUIMARAES A R,et al.Imaging primary lung cancers in mice to study radiation biology[J]. Int J Radiat Oncol Biol Phys,2010,76(4):973-977.
[11] DUTT A,WONG K K.Mouse models of lung cancer[J]. Clin Cancer Res,2006,12(14):4396-4402.
[12] SHMIDT E N,NITKIN A Y.Pathology of mouse models of human lung cancer[J].Comp Med,2004,54(1):23-26.
[13] MEUWISSEN R.Mouse models for human lung cancer[J]. Genes & Development,2005,19:643-664.
[14] MALKINSON A M.Primary lung tumors in mice as an aid of understanding,preventing and treating human adenocarcinoma of the lung[J]. Lung Cancer,2001,32(3):265-269.
[15] 余涛,叶坚. 高效液相色谱同时测定食品中BHA、BHT、TBHQ 方法的研究[J]. 中国卫生检验杂志,2007,17(12):2136-2137.
[16] 周宗灿,毒理学基础[M]. 2版. 北京:北京医科大学出版社,2007:124.
[17] 李寿祺. 毒理学原理与方法[M]. 2版. 成都:四川大学出版社,2003:195-197.
[18] WITSCHI H R,MORSE C C.Enhance of lung tumor formation in mice by dietary butylated hydroxytoluene:dose-time relationships and cell kinetics[J]. JNCI,1983,71(4):859-866.
[19] WITSCHI H P.Promotion of lung tumors in mice.[J]. Environmental Health Perspectives,1983,50:267-273.
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