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| Regulatory effects of lactoferrin and 2'-fucosyl-lactose complex powder on immune function in mice |
| CAI Delei, SONG Yanhua, CAO Bin, ZHAO Lili, LU Wei
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| Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China |
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Abstract Objective To explore the immunomodulatory effect of lactoferrin and 2'-fucosyl-lactose (2'-FL) compound powder on immune function in mice. Methods Forty female ICR mice were randomly divided into a control group and three dose groups (0.125, 0.250, and 0.750 g/kg body weight), with 10 mice in each group. The three dose groups were intragastrically administered the compound powder solution at 10 mL/kg body weight daily, while the control group received an equal volume of deionized water for 30 consecutive days. The splenic lymphocyte transformation test and delayed-type hypersensitivity test were used to measure the optical density difference and the left hind paw thickness difference, respectively. Serum hemolysin assay and antibody-forming cell assay were performed to determine the antibody volume and the number of hemolytic plaques. The peritoneal macrophage phagocytosis assay with chicken red blood cells was conducted to calculate the phagocytosis rate and phagocytic index. The natural killer (NK) cell activity assay was used to measure optical density and calculate NK cell activity. Results Compared with the control group, the optical density difference and left hind paw thickness difference were significantly increased in the high-dose group, the peritoneal macrophage phagocytosis rate of chicken red blood cells was significantly increased in the medium-dose and high-dose groups, awhile the phagocytic index was significantly elevated in the medium-dose group (all P<0.05). There were no significant differences in antibody volume, hemolytic plaque number or NK cell activity among the four groups (all P>0.05). Conclusion Lactoferrin and 2'-FL compound powder exerts an immunomodulatory effect by enhancing cellular immunity and monocyte-macrophage function.
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Received: 23 January 2026
Revised: 24 April 2026
Published: 27 July 2026
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