|
|
|
| Genomic characteristics of Salmonella among diarrhea cases in Hohhot City |
| WANG Yanfei1, XING Liping1, DU Xiaoli2, CHEN Yanying2, YANG Hairong1, XING Fangchao1, LI Ping1, CUI Zhigang2
|
1. Hohhot Center for Disease Control and Prevention (Hohhot Institute of Public Health Supervision), Hohhot, Inner Mongolia 010020, China;
2. Chinese Center for Disease Control and Prevention, Beijing 102206, China |
|
|
|
|
Abstract Objective To analyze the whole genome characteristics of Salmonella in diarrhea cases in Hohhot City, so as to provide the evidence for the prevention and control of Salmonella infection and rational clinical use of antibiotics. Methods Salmonella strains were isolated and identified from fecal specimens of acute diarrhea cases at sentinel hospitals for national foodborne disease surveillance in Hohhot City from 2021 to 2023. DNA was extracted for whole genome sequencing. Bioinformatics analysis based on the Microobench Pathogen Genome Analysis System was conducted to determine serotypes, multi-locus sequence typing (MLST), drug resistance genes, and virulence genes. Additionally, 60 Salmonella genomic sequences from different regions across China were downloaded from the NCBI Genome database. A phylogenetic tree was constructed based on core-genome MLST (cgMLST) to analyze the phylogenetic relationship between strains from Hohhot City and those from other regions of China. Results From 2021 to 2023, a total of 42 Salmonella strains isolated in Hohhot City were classified into 12 serotypes. Salmonella Enteritidis ST11, Salmonella I 4,[5],12:i:- ST34, and Salmonella Typhimurium ST19 were identified as the dominant strains, represented by 13, 10, and 6 isolates, which accounted for 30.95%, 23.81%, and 14.29%, respectively. Seventy-seven drug resistance genes belonging to 12 classes were detected. Among them, 17 Salmonella strains carried amino acid substitutions in the fluoroquinolone resistance-determining region caused by mutations at the gyrA and parC loci. Virulence genes covered all stages of Salmonella pathogenesis, with a predominance of genes associated with adhesion. Phylogenetic tree analysis results revealed that the 42 Salmonella strains from Hohhot City were distributed across evolutionary branches A, B, C, D, and F. They clustered with 60 strains from other regions of China, suggesting potential cross-regional transmission. Conclusions Salmonella strains isolated in Hohhot City exhibited diverse serotypes, carried multiple drug resistance genes and virulence genes, and displayed high genetic diversity. These findings indicate a potential risk of cross-regional transmission, highlighting the need for enhanced surveillance and prevention measures.
|
|
Received: 03 November 2025
Revised: 06 January 2026
Published: 11 February 2026
|
|
|
|
|
|
[1] 刘理慧,储锦华,隋雨欣,等.沙门氏菌中主要毒力因子的研究进展[J].生物技术通报,2022,38(9):72-83.
[2] 罗丹丹,鲁志平,文永平,等.2012—2024年我国部分地区禽源沙门氏菌耐药情况研究进展[J].中国动物传染病学报,2025,33(2):178-193.
[3] 林旭东. 消毒剂胁迫下猪沙门氏菌对抗生素耐药性的共筛选机制[D].武汉:华中农业大学,2024.
[4] 高瑞霞,滕贇,张彦斌,等.内蒙古呼和浩特地区鸡源沙门氏菌分离鉴定、血清学鉴定及耐药性研究[J].食品安全质量检测学报,2019,10(6):1705-1709.
[5] 周虓,李萍,罗云,等.2013—2014年呼和浩特市食源性疾病致病菌监测分析[J].疾病监测与控制,2015,9(6):412-413.
[6] XIE J M,CHEN Y R,CAI G J,et al.Tree Visualization By One Table(tvBOT):a web application for visualizing,modifying and annotating phylogenetic trees[J].Nucleic Acids Res,2023,51(W1):587-592.
[7] 严伟,查赟峰,纪蕾,等.2018—2020年湖州市食源性疾病患者感染沙门菌耐药与分子分型研究[J].中国预防医学杂志,2022,23(8):622-627.
[8] 王振宇. 鼠伤寒沙门菌单相变异体的微演化特征和产生机制研究[D].扬州:扬州大学,2024.
[9] 杨毅,陈国利,胡锦瑞,等.乌鲁木齐市鼠伤寒沙门菌单相变种1,4,[5],12:i:-基因组特征[J].中国热带医学,2025,25(10):1316-1322.
[10] 李伟. mcr-1基因在食源性沙门氏菌及大肠杆菌中的转移机制[D].杨凌:西北农林科技大学,2021.
[11] QU M,HUANG Y,LYU B,et al.Prevalence and genomic characterization of multidrug-resistant Salmonella enterica serovar Kentucky sequence type 198 circulating-Beijing Municipality,China,2016-2023[J].China CDC Weekly,2024,6(33):825-833.
[12] 蒋培余,潘劲草.细菌遗传元件水平转移与抗生素抗性研究进展[J].微生物学通报,2006,33(4):167-171.
[13] LOU L X,ZHANG P,PIAO R L,et al.Salmonella pathogenicity island 1(SPI-1)and its complex regulatory network[J/OL].Front Cell Infect Microbiol,2019,9[2026-01-06].https://doi.org/10.3389/fcimb.2019.00270.
[14] 邵增玉. 沙门氏菌E3泛素连接酶SspH2和SlrP调控Ⅰ型IFN信号通路的研究[D].北京:中国农业科学院,2019.
[15] 顾宣强,侯千禧,刘家奇,等.肠炎沙门菌sef14基因缺失株生物学功能[J].中国兽医学报,2022,42(1):71-77. |
|
|
|
