Abstract:Objective To investigate the rodent density and species composition in Wenzhou City, Zhejiang Province from 2020 to 2024, so as to provide evidence for formulating rodent prevention and control measures. Methods From 2020 to 2024, all counties (cities, districts) in Wenzhou City were selected as monitoring sites. The trap-night method, cage-night method and sticky mouse board method were used to monitor rodents in urban residential areas, key industries, and rural residential areas. The rodent density and population composition in different years, habitats and monitoring methods were analyzed, and the average annual percent change (AAPC) was used to analyze the changing trend of rodent density from 2020 to 2024. Results A total of 2 022 rodents were captured in Wenzhou City from 2020 to 2024, with an average rodent density of 0.96%. The numbers of captured rodents in each year were 384, 424, 365, 460 and 389, and the corresponding rodent densities were 0.89%, 1.01%, 0.84%, 1.05% and 1.02%, respectively. The difference in rodent density among years showed statistical significance (P<0.05), while the temporal trend showed no statistical significance (AAPC = 3.162%, P>0.05). The captured rodent species included Rattus norvegicus, Mus musculus and Rattus tanezumi, with 1 227, 486 and 309 captured, accounting for 60.68%, 24.04% and 15.28%. From 2020 to 2024, 742, 659 and 621 rodents were captured in urban residential areas, key industries and rural residential areas, with rodent densities of 1.07%, 0.94% and 0.87%, respectively, and the difference showed statistical significance (P<0.05). A total of 812, 394 and 816 rodents were captured by the trap-night method, cage-night method and sticky mouse board method, with rodent densities of 1.06%, 0.72% and 1.03%, respectively and; the difference showed statistical significance (P<0.05). Conclusions The temporal trend of rodent density remained stable in Wenzhou City from 2020 to 2024. Rattus norvegicus was the dominant rodent species. The rodent density was relatively higher in urban residential areas and monitored by the trap-night method.