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预防医学  2021, Vol. 33 Issue (8): 772-776    DOI: 10.19485/j.cnki.issn2096-5087.2021.08.004
  论著 本期目录 | 过刊浏览 | 高级检索 |
碱性饮用水预防小鼠高尿酸血症的作用研究
于莎莉1, 林晨2, 蒋志韬3, 朱超4, 赵新元1
1. 南通大学公共卫生学院环境卫生学教研室,江苏 南通 226019;
2. 上海市浦东新区疾病预防控制中心病媒生物与寄生虫病防治科;
3. 中国计量大学机电工程学院机械设计制造及其自动化系;
4. 海军军医大学第三附属医院肾内科
Preventive effect of alkaline drinking water on hyperuricemia in mice
YU Shali*, LIN Chen, JIANG Zhitao, ZHU Chao, ZHAO Xinyuan
*Department of Environmental Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu 226019, China
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摘要 目的 观察碱性饮用水预防小鼠高尿酸血症的作用。方法 60只雄性SPF级昆明小鼠随机分为6组:碱性饮用水干预组(包括pH 7.3、pH 8.0、pH 9.3饮用水干预组),分别饮用pH值为7.3±0.5、8.0±0.5和9.3±0.6的饮用水;对照组、模型组和阳性药物组(别嘌醇终浓度为2 g/L)饮用普通双蒸水。除对照组外,各组小鼠连续酵母(1.5 g/mL)灌胃13 d,第14天腹腔注射300 mg/kg的氧嗪酸钾后禁食不禁水1 d。第16天取血清检测尿酸、肌酐和尿素氮,取肾脏组织切片染色后观察形态,采用蛋白质免疫印迹法检测肾脏组织中性粒细胞明胶酶相关载脂蛋白(NGAL)、金属蛋白酶组织抑制因子1(TIMP1)、有机阴离子转运蛋白1(OAT1)、尿酸盐转运蛋白1(URAT-1);采用实时荧光定量PCR检测URAT-1和OAT1的mRNA。结果 模型组小鼠血清尿酸含量高于对照组和pH 9.3饮用水干预组(均P<0.05)。pH 9.3饮用水干预组小鼠肾小管病变个数和肾小管病变面积少于模型组(均P<0.05)。pH 9.3饮用水干预组小鼠NGAL和URAT-1蛋白相对表达量低于模型组,OAT1蛋白相对表达量高于模型组(均P<0.05)。pH 9.3饮用水干预组小鼠URAT-1 mRNA的相对表达量低于模型组,OAT1 mRNA的相对表达量高于模型组(均P<0.05)。结论 pH值为9.3±0.6的碱性饮用水能有效预防小鼠高尿酸血症及其引起的急性肾脏组织损伤。
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于莎莉
林晨
蒋志韬
朱超
赵新元
关键词 高尿酸血症碱性水小鼠尿酸肾脏    
AbstractObjective To observe the preventive effect of alkaline drinking water on hyperuricemia in mice. Methods Sixty male SPF Kunming mice were randomly divided into six groups: pH 7.3, pH 8.0, pH 9.3 intervention groups, in which the mice were given water with pH values of 7.3±0.5, 8.0±0.5 and 9.3±0.6, respectively; the control group, model group and positive drug group ( with 2 g/L allopurinol ) were given double distilled water. Except for the control group, the mice in each group were given yeast by gavage ( 1.5 g/mL ) for 13 days. On the 14th day, the mice were injected with 300 mg/kg potassium oxyzinate by intraperitoneal injection, and then fasted for 1 day. On the 16th day, serum uric acid, creatinine and urea nitrogen were detected, and renal tissues were stained to observe the morphology. The expression levels of neutrophil gelatinase-associated lipocalin ( NGAL ), tissue inhibitor of metalloproteinase 1
Key wordshyperuricemia    alkaline water    mice    uric acid    kidney
收稿日期: 2022-09-04      出版日期: 2021-08-10
ZTFLH:  R589.7  
基金资助:江苏省高校自然科学研究面上项目(16KJB330007)
通信作者: 赵新元,E-mail:zhaoxinyuan@ntu.edu.cn   
作者简介: 于莎莉,博士,讲师,主要从事环境污染物与人群健康相关研究工作
引用本文:   
于莎莉, 林晨, 蒋志韬, 朱超, 赵新元. 碱性饮用水预防小鼠高尿酸血症的作用研究[J]. 预防医学, 2021, 33(8): 772-776.
YU Shali, LIN Chen, JIANG Zhitao, ZHU Chao, ZHAO Xinyuan. Preventive effect of alkaline drinking water on hyperuricemia in mice. Preventive Medicine, 2021, 33(8): 772-776.
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