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预防医学  2017, Vol. 29 Issue (7): 680-684    DOI: 10.19485/j.cnki.issn1007-0931.2017.07.007
  论著 本期目录 | 过刊浏览 | 高级检索 |
葫芦素B对脂多糖诱导小鼠肺泡巨噬细胞炎症反应的影响
周康, 张永为, 陆新建, 马春芳
浙江省新华医院检验科,浙江 杭州 310005
A study on the effect of Cucurbitacin B on the inflammatory reaction of murine alveolar macrophages induced by lipopolysaccharide
ZHOU Kang, ZHANG Yong-wei, LU Xin-jian, MA Chun-fang
Xinhua Hospital of Zhejiang Province,Hangzhou,Zhejiang,310005,China
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摘要 目的了解葫芦素B(CuB)对脂多糖(LPS)诱导小鼠肺泡巨噬细胞炎症反应的影响及其分子机制。方法将小鼠肺泡巨噬细胞分为对照组、LPS组和LPS+不同浓度CuB组,对照组不作任何处理,LPS组用1μg/mL LPS诱导,另外3组加入不同浓度CuB使浓度分别为1、2.5和5 μM,培养2 h后用1 μg/mL LPS诱导。采用CCK-8法检测细胞活性,ELISA法检测细胞因子(TNF-α、IL-1β、IL-6、PGE2)水平,Griess Reagent法检测NO浓度,WB法检测iNOS、COX-2、Nrf2和HO-1的蛋白表达水平。结果分组培养24 h后各组细胞活性差异无统计学意义(P>0.05),本实验浓度下的LPS和CuB对细胞无明显毒性。LPS组细胞上清液中细胞因子和NO水平均高于对照组(P<0.05);经CuB处理的LPS组细胞上清液中炎症因子浓度较LPS组明显降低(均P<0.05),并呈剂量依赖性。LPS刺激后,细胞中iNOS、COX-2、Nrf-2和HO-1表达量均增加,CuB可抑制iNOS和COX-2的表达,促进Nrf-2和HO-1的表达。结论CuB能抑制LPS诱导后巨噬细胞炎症因子的释放,作用机制可能是抑制iNOS和COX-2表达,促进Nrf-2和HO-1表达。
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周康
张永为
陆新建
马春芳
关键词 葫芦素B脂多糖巨噬细胞炎症因子    
Abstract:Objective To investigate the anti-inflammatory effect of Cucurbitacin B on the inflammatory reaction of murine alveolar macrophages induced by lipopolysaccharide and its molecular mechanism. Methods The alveolar macrophages were randomly divided into five groups: blank group, LPS group, different concentrations of Cucurbitacin B (1μM, 2.5μM, 5μM)+) +LPS groups. All group cells were pretreated with different concentrations for 2 h, and followed by 1μg/mL LPS. The protein level of TNF-α,IL-1β,IL-6 and PGE2 were detected by ELISA assay, and the NO was detected by Griess methods, and its upstream protein i-NOS,COX-2, Nrf2 and HO-1 were detected by Western-blot. Results The secretion of TNF-α,IL-1β,IL-6 and PGE2 in LPS group were increased significantly than blank groups, while Cucurbitacin B suppressed its protein level inadose-dependent manner. The nuclear erythroid related factor 2 and antioxidant gene were activated while Cucurbitacin B was added into alveolar macrophages in adose-dependent manner. Conclusion Cucurbitacin B can inhibit the extracellar secretion of inflammatory cytokines production, and its molecular mechanism could be due to the promoting Nrf2 translocation to the nucleus, activating the expression of HO-1 gene, reducing the expression of iNOS and COX-2, and thereby alleviating the inflammatory reaction.
Key wordsCucurbitacin B    Lipopolysaccharide    Macrophage-inflammatory cytokine
收稿日期: 2017-01-04      出版日期: 2017-11-15
ZTFLH:  R392.1  
通讯作者: 马春芳,E-mail:zjusoso@aliyun.com   
作者简介: 周康,本科,初级检验技师,主要从事临床检验工作
引用本文:   
周康, 张永为, 陆新建, 马春芳. 葫芦素B对脂多糖诱导小鼠肺泡巨噬细胞炎症反应的影响[J]. 预防医学, 2017, 29(7): 680-684.
ZHOU Kang, ZHANG Yong-wei, LU Xin-jian, MA Chun-fang. A study on the effect of Cucurbitacin B on the inflammatory reaction of murine alveolar macrophages induced by lipopolysaccharide. Preventive Medicine, 2017, 29(7): 680-684.
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http://www.zjyfyxzz.com/CN/10.19485/j.cnki.issn1007-0931.2017.07.007      或      http://www.zjyfyxzz.com/CN/Y2017/V29/I7/680
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