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预防医学  2020, Vol. 32 Issue (10): 983-986    DOI: 10.19485/j.cnki.issn2096-5087.2020.10.003
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阜新市HIV-1env基因C2~V4区序列特征分析
李贺1, 杜波1, 田晓东1, 刘文新1, 姚文清2
1.阜新市卫生健康服务中心,辽宁 阜新 123000;
2.辽宁省疾病预防控制中心
Sequence analysis of C2-V4 of HIV-1 env genes in Fuxin
LI He*, DU Bo, TIAN Xiaodong, LIU Wenxin, YAO Wenqing
*Fuxin Health Service Center, Fuxin, Liaoning 123000, China
全文: PDF(867 KB)  
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摘要 目的 分析阜新市艾滋病病毒1型(HIV-1)env基因C2~V4区序列及代表性广谱单克隆中和抗体的表位变异,为HIV-1变异趋势研究及阐明V3环与病毒生物学特征提供依据。方法 采集2018—2019年在阜新市卫生健康服务中心确证阳性的112例HIV-1感染者全血标本,采用巢式PCR扩增env基因C2~V4区核苷酸序列并测序,采用MEGA软件鉴定分型,采用HIV Database等软件分析V3环顶端四肽、辅助受体、净电荷和特征性氨基酸。结果 获得101条有效基因序列,发现5种V3环顶端四肽类型,其中GPGQ 77条,占76.24%,存在于CRF01_AE、CRF07_BC、CRF65_cpx和G亚型;GPGR 19条,占18.81%,存在于CRF01_AE、CRF07_BC和B亚型;GPGH 3条,GPGK和GPGA 各1条,均只存在于CRF01_AE亚型。辅助受体主要使用CCR5,84条占83.17%。CRF01_AE、CRF07_BC、B、CRF65_cpx和G亚型V3环净电荷数分别为3.28±1.17、3.22±0.92、4.25±0.83、2.50±0.50和3。结合b12和VRC01中和抗体表位氨基酸位点突变率为0~9.90%;295位和332位氨基酸N-糖基化位点缺失率分别为18.81%和14.85%,未发现两者同时缺失的情况。结论 2018—2019年阜新市HIV-1毒株主要是以CCR5为辅助受体的巨噬细胞嗜性非合胞体诱导型病毒,V3环顶端四肽以GPGQ为主,复制速度较慢,逃逸中和抗体能力较低。
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李贺
杜波
田晓东
刘文新
姚文清
关键词 艾滋病病毒1型env基因变异包膜蛋白V3环    
AbstractObjective To learn the sequence characteristics of C2-V4 of HIV-1 env genes and the epitope variation of representative broadly monoclonal neutralizing antibody in Fuxin, so as to provide evidence for the HIV-1 variation trend and the biological characteristics of V3 loop. Methods The whole blood samples of 112 HIV-1 cases in Fuxin Health Service Center from 2018 to 2019 were collected and the DNA was extracted. The C2-V4 of env genes were amplified by nested-PCR and the PCR products were subjected to sequencing. The bioinformatics analysis was carried out using MEGA software, and the V3-tip motifs, co-receptors, net charge and characteristic amino acids were analyzed using HIV Database. Results Totally 101 effective gene sequences were obtained, and 5 types of V3-tip motifs were found. Among them, 77 pieces of GPGQ ( 76.24% ) were found in CRF01_AE, CRF07_BC, CRF65_cpx and G subtypes; 19 pieces of GPGR ( 18.81% ) were found in CRF01_AE, CRF07_BC and B subtypes; 3 pieces of GPGH, 1 piece of GPGK and 1 piece of GPGA were only found in CRF01_ AE subtype. The co-receptor was mainly CCR5 ( 84, 83.17% ) . The net charge numbers of V3 loops in CRF01_ AE, CRF07_ BC, B, CRF65_cpx and G were 3.28±1.17, 3.22±0.92, 4.25±0.83, 2.50±0.50 and 3, respectively. The mutation rates of neutralizing antibodies binding b12 and VRC01 were 0-9.90%. The deletion rates of N-glycosylation sites of 295 and 332 were 18.81% and 14.85%, without the loss of both sites. Conclusions The HIV-1 strains in Fuxin from 2018 to 2019 are macrophage-tropic and non-syncytium-inducing, with GPGQ as the main type of V3-tip motif, CCR5 as the main co-receptor, slow replication and low ability to escape neutralizing antibodies.
Key wordsHIV-1    env genes    mutation    envelop protein    V3 loop
收稿日期: 2020-05-25      出版日期: 2020-09-30
ZTFLH:  R512.91  
基金资助:国家科技重大专项艾滋病和病毒性肝炎等重大传染病防治项目(2017ZX10103007)
通信作者: 田晓东,E-mail:dt520@fxcdc.org   
作者简介: 李贺,硕士,主管检验师,主要从事实验室检测工作
引用本文:   
李贺, 杜波, 田晓东, 刘文新, 姚文清. 阜新市HIV-1env基因C2~V4区序列特征分析[J]. 预防医学, 2020, 32(10): 983-986.
LI He, DU Bo, TIAN Xiaodong, LIU Wenxin, YAO Wenqing. Sequence analysis of C2-V4 of HIV-1 env genes in Fuxin. Preventive Medicine, 2020, 32(10): 983-986.
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http://www.zjyfyxzz.com/CN/10.19485/j.cnki.issn2096-5087.2020.10.003      或      http://www.zjyfyxzz.com/CN/Y2020/V32/I10/983
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