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预防医学  2019, Vol. 31 Issue (11): 1086-1090    DOI: 10.19485/j.cnki.issn2096-5087.2019.11.002
  论著 本期目录 | 过刊浏览 | 高级检索 |
秀丽隐杆线虫模型评估威尼斯不动杆菌的致病性
李煜1,2,王雪岩2,胡晓珂3,杨梦迪1,罗环1,梁婧1,李隆飞1,赵金萍1,毕洁1 ,陈斌1,王如刚1
1.北京市疾病预防控制中心职业健康体检中心,北京 100020;
2.首都医科大学公共卫生学院;
3.中国科学院烟台海岸带研究所海岸带生物学与生物资源利用重点实验室
Evaluation of pathogenicity of Acinetobacter venetum by using Caenorhabditis elegans
LI Yu*,WANG Xue-yan,HU Xiao-ke,YANG Meng-di,LUO Huan,LIANG Jing,LI Long-fei,ZHAO Jin-ping,BI Jie,CHEN Bin,WANG Ru-gang
*Occupational Health Examination Center,Beijing Center for Disease Control and Prevention,Beijing 100020,China
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摘要 目的 应用秀丽隐杆线虫模型评估可能用作环境修复剂的威尼斯不动杆菌(Av)的致病性。方法 分别在铺有大肠杆菌OP50和Av的培养基上培养秀丽隐杆线虫,通过观察秀丽隐杆线虫的发育、运动、消化功能、寿命和繁殖能力评估Av致病性;同时按照NY 1109—2017《微生物肥料生物安全通用技术准则》进行Av致病性试验,比较两种方法的评估结果。结果 应用秀丽隐杆线虫模型发现,Av组与OP50组成年秀丽隐杆线虫的体长、体宽、头部摆动频率和身体弯曲频率差异均无统计学意义(P>0.05);Av组平均排便循环时间缩短(P<0.05);Av组和OP50组线虫的生存曲线基本重合,平均寿命分别为(13.5±0.4)d和(13.7±0.4)d,差异无统计学意义(P>0.05);Av组后代总数比OP50组增加了18.7%。NY 1109—2017《微生物肥料生物安全通用技术准则》评估体系发现,雌性、雄性小鼠急性经口LD50均>10 g/kgbw,属实际无毒;急性腹腔注射致病性试验结果显示动物未出现中毒体征及死亡,大体解剖未见异常;Av对家兔破损皮肤和眼均为无刺激性;溶血试验结果为阴性;Av对7种不同类别的抗菌药物均敏感,对1种抗菌药物中介。结论 Av无致病性;采用秀丽隐杆线虫模型评价环境修复剂的致病性具有可行性,可考虑作为致病性评价体系的筛选方案。
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李煜
王雪岩
胡晓珂
杨梦迪
罗环
梁婧
李隆飞
赵金萍
毕洁
陈斌
王如刚
关键词 秀丽隐杆线虫;威尼斯不动杆菌;致病性    
AbstractObjective To evaluate the pathogenicity of Acinetobacter venetum(Av),which is expected to be used as an environmental remediation agent,using Caenorhabditis elegans(C.elegans). Methods The C.elegans were cultured on the media loaded with E.coli OP50 and Av,respectively. The pathogenicity of Av was evaluated by observing the effects of Av on the growth,movement,digestive function,lifespan and reproduction of C.elegans,compared with that of another evaluation system according to NY 1109-2017 General Biosafety Standard for Microbial Fertilizers. ResultsBy C. elegans system,it was found that the body length,width,head thrash frequency,body bending frequency and average lifespan [(13.5±0.4)d vs.(13.7±0.4)d] of adult nematodes in the Av group were not significantly different from those in the OP50 group(all P>0.05);while the average time of defecation cycle in the Av group shortened,the total number of progenies in the Av group increased by 18.7%(all P<0.05). According to NY1109-2017 General Biosafety Standard for Microbial Fertilizers,it was found that the oral LD50 values for both male and female mice were more than 10 g/kgbw,which was practically non-toxic;the pathogenicity test of acute intraperitoneal injection showed that the animals did not have signs of poisoning,deaths or any abnormalities in gross anatomy;Av had no irritation to damaged skin and eyes of rabbits;the hemolysis test was negative;Av was sensitive to seven antibiotics and was medium to one antibiotic. Conclusion Av is not pathogenic. C. elegans can be used in early screening for the pathogenicity of environmental remediation agents.
Key wordsAcinetobacter venetum    Caenorhabditis elegans    Pathogenicity
          出版日期: 2019-11-01
中图分类号:  R37  
基金资助:国家自然科学基金(41576156);北京市自然科学基金(7152024);北京市委组织部优秀人才培养项目(2012A003034000014)
通信作者: 王如刚,E-mail:bjcdczw@163.com   
作者简介: 李煜,硕士,副教授,主要从事模式生物毒理学和职业健康检测研究工作
引用本文:   
李煜,王雪岩,胡晓珂,杨梦迪,罗环,梁婧,李隆飞,赵金萍,毕洁 ,陈斌,王如刚. 秀丽隐杆线虫模型评估威尼斯不动杆菌的致病性[J]. 预防医学, 2019, 31(11): 1086-1090.
LI Yu,WANG Xue-yan,HU Xiao-ke,YANG Meng-di,LUO Huan,LIANG Jing,LI Long-fei,ZHAO Jin-ping,BI Jie,CHEN Bin,WANG Ru-gang. Evaluation of pathogenicity of Acinetobacter venetum by using Caenorhabditis elegans. Preventive Medicine, 2019, 31(11): 1086-1090.
链接本文:  
http://www.zjyfyxzz.com/CN/10.19485/j.cnki.issn2096-5087.2019.11.002      或      http://www.zjyfyxzz.com/CN/Y2019/V31/I11/1086
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