Abstract:Objective To evaluate the immune function of Dendrobium and Ganoderma lucidum oral liquid in female KM mice. Methods A total of 160 healthy female KM mice were selected and divide into three dose groups and a control group. Dendrobium and Ganoderma lucidum oral liquid was administered to the mice in the three dose groups at 3.33,6.67 and 10.00 mL/kgbw by intragastric lavage for 30 days,while distilled water was given to the mice in the control group in the same way. The thymus and spleen were weighed and the visceral organ coefficients were calculated. The humoral immune function,cellular immune function,monocyte-macrophage phagocytosis function and natural killer(NK)cell activity were detected,then the effects of the oral liquid on immunity enhancement of mice could be proved with any two items above positive. Results No statistically significant differences were found between the dose groups and the control group in the visceral organ coefficients,serum hemolysis,lymphocyte proliferation ability,macrophage phagocytosis and NK cell activity(P>0.05). In the middle- and high-dose group,the number of plague-forming cells per 106 spleen cells were 315.5±49.9 and 343.0±43.8,respectively,which were more than that in the control group(249.5±41.3,P<0.05);the capacity of toe swelling in the middle- and high-dose group were(1.34±0.42)and(1.37±0.49)mm,respectively,which were more than that in the control group [(0.89±0.37)mm,P<0.05]. Conclusion Dendrobium and Ganoderma lucidum oral liquid have the effect of enhancing immunity on female KM mice.
[1] 王洪云,李铭. 石斛多糖的药用功能研究进展[J]. 环球中医药,2014,7(10):817-820. [2] 陈玉满,鹿伟,傅剑云,等. 铁皮石斛西洋参混合物对不同性别小鼠缓解体力疲劳作用的研究[J]. 浙江预防医学,2011,23(10):19-21,30. [3] 鹿伟,陈玉满,徐彩菊,等 . 铁皮石斛抗疲劳作用研究[J]. 中国卫生检验杂志,2010,20(10):2488-2490. [4] LIN X,SHAW P C,SZE S C,et al.Dendrobium officinale polysaccharides ameliorate the abnormality of aquaporin 5,pro-inflammatory cytokines and inhibit apoptosis in the experimental Sj?gren's syndrome mice[J].Int Immunopharmacol,2011,11(12):2025-2032. [5] 张娥珍,黄梅华,辛明,等. 铁皮石斛纳米粉与超微粉的物理特性和体外抗氧化活性比较研究[J]. 热带作物学报,2015,36(12):2184-2191. [6] 王再花,章金辉,李杰,等 . 3种药用石斛多糖的抗氧化活性比较研究[J]. 华南师范大学学报(自然科学版),2015,47(5):65-70. [7] JIN L H,LIU C F,TANG T,et al.Experimental study on anti-tumor effect of Dendrobium candidum polysaccharides[J]. Chin Pharm J,2010,45(22):1734-1737. [8] MENG L Z,LYU G P,HU D J,et al.Effects of polysaccharides from different species of Dendrobium(Shihu)on macrophage function[J]. Molecules,2013,18(5):5779-5791. [9] 李光,宋美芳,李宜航,等. 不同种类石斛多糖成分对小鼠脾脏免疫功能的影响[J]. 中国临床药理学与治疗学,2012,17(10):1108-1111. [10] 施仁潮,竹剑平,李明焱,等. 铁皮石斛抗肿瘤作用的研究进展[J]. 中国药学杂志,2013,48(19):1641-1644. [11] 李伟,张静,周雯,等. 铁皮石斛对免疫抑制小鼠的免疫调节作用和血清细胞因子的影响[J]. 卫生研究,2016,45(1):137-139. [12] 国家药典委员会.中华人民共和国药典 2015年版[M].北京:中国医药科技出版社,2015:92-93,188-189. [13] WANG P Y,ZHU X L,LIN Z B.Antitumor and immunomodulatory effects of polysaccharides from broken-spore of Ganoderma lucidum[J]. Front Pharmacol,2012,3:135. [14] 陈成章. 免疫毒理学[M].郑州:郑州大学出版社,2008:430-442. [15] 段立群,姚晓东,张渊,等. 石斛复方制剂对小鼠免疫功能的影响[J] . 遵义医学院学报,2015,38(1):41-44. [16] 徐海军,方予,汪俊涛,等. 霍山石斛多糖对小鼠的双向免疫调节作用[J] . 免疫学杂志,2018,34(8):731-736. [17] 李妮亚,高培元,王紫.海南石斛属和金钗石斛属植物多糖及氨基酸含量分析[J].植物资源与环境学报,2004,13(4):57-58. [18] 宋燕华,蔡德雷,傅剑云,等. 铁皮石斛叶对二代繁殖雌性大鼠免疫水平影响的研究[J]. 浙江预防医学,2016,28(2):109-112.