Effect of aluminum exposure on abnormal phosphorylationof tau protein in PC12 cells of rats
SONG Shanshan1, XU Xu1, XU Yangdan2, XIAO Wenjie1, YANG Xiaojuan1,2
1. School of Public Health, Shanxi Medical University, Taiyuan, Shanxi 030001, China; 2. The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China
Abstract：Objective To investigate the effect of aluminum exposure on expression of miR-497-5p, wingless murine breast cancer virus integration site family member 3a (Wnt3a), β-catenin protein, glycogen synthase kinase-3β (GSK-3β) protein and tau protein in rat adrenal pheochromocytoma PC12 cells, so as to provide insight into unraveling the mechanisms underlying aluminum exposure-induced abnormal phosphorylation of tau protein. Methods PC12 cells were exposed to Al(mal)3 at concentrations of 0, 100, 200, 400 μmol/L for 24 h. The viability of PC12 cells was measured using cell counting kit-8 (CCK-8) assay. The relative expression of miR-497-5p and Wnt3a was detected using a real-time fluorescent quantitative PCR (RT-qPCR) assay, and the expression of Wnt3a, β-catenin, GSK-3β, P-GSK-3β (Ser9), tau and p-tau (Ser396) proteins were determined using Western blotting. Results The viability of PC12 cells appeared a tendency towards a decline with the increase of aluminum dose (Ftrend=323.473, P=0.001). RT-qPCR assay detected that the relative miR-497-5p expression appeared a tendency towards a rise with the increase of aluminum dose (Ftrend=14.888, P=0.031), and the relative Wnt3a expression appeared a tendency towards a decline with the increase of aluminum dose (Ftrend=165.934, P<0.001). The miR-497-5p expression negatively correlated with the relative Wnt3a expression (r=-0.693, P=0.012). The expression of Wnt3a (Ftrend=357.656, P=0.001), β-catenin (Ftrend=208.750, P=0.001) and p-GSK-3β (Ser9) proteins (Ftrend=512.583, P<0.001) appeared a tendency towards a decline with the increase of aluminum dose, and the expression of GSK-3β (Ftrend=39.965, P<0.001), tau (Ftrend=277.929, P=0.006) and p-tau (Ser396) proteins (Ftrend=96.247, P=0.002) appeared a tendency towards a rise with the increase of aluminum dose. Conclusion Up-regulation of miR-497-5p and GSK-3β expression and down-regulation of Wnt3a and β-catenin expression may be a mechanism underlying aluminum exposure-induced abnormal phosphorylation of tau protein.
宋珊珊, 徐旭, 许杨丹, 肖雯洁, 杨晓娟. 铝暴露对大鼠PC12细胞tau蛋白异常磷酸化的影响[J]. 预防医学, 2023, 35(3): 271-274.
SONG Shanshan, XU Xu, XU Yangdan, XIAO Wenjie, YANG Xiaojuan. Effect of aluminum exposure on abnormal phosphorylationof tau protein in PC12 cells of rats. Preventive Medicine, 2023, 35(3): 271-274.
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