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预防医学  2021, Vol. 33 Issue (6): 573-578    DOI: 10.19485/j.cnki.issn2096-5087.2021.06.007
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2,2',4,4'-四溴联苯醚对小鼠3T3-L1细胞分化的影响研究
海且木汗·阿布杜热曼, 阿依古丽·阿力木, 李美艳, 王永治, 王嘉穗, 刘早玲
新疆医科大学公共卫生学院,新疆 乌鲁木齐 830011
Effects of 2,2',4,4' -tetrabromodiphenyl ether on differentiation of mouse 3T3-L1 cells
Haiqiemuhan Abudureman, Ayiguli Alimu, LI Meiyan, WANG Yongzhi, WANG Jiasui, LIU Zaoling
School of Public Health, Xinjiang Medical University,Urumqi,Xinjiang 830011,China
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摘要 目的 研究2,2',4,4'-四溴联苯醚(BDE-47)对小鼠胚胎成纤维细胞3T3-L1分化的影响,为揭示环境肥胖因素的作用规律提供依据。方法 将3T3-L1细胞分为5组BDE-47干预组(25、18.75、12.5、7.5、2.5 μmol/L)、阳性对照组(加1 μmol/L 2, 4-噻唑烷二酮)和阴性对照组(加0.1%二甲基亚砜),诱导分化第8 d油红O染色处理并检测吸光度值(OD)观察脂肪细胞脂滴聚集情况;采用三酰甘油(TG)酶法测定细胞培养液的TG含量;采用RT-PCR法测定脂联素和过氧化物酶体增殖子激活受体γ(PPARγ)的mRNA表达。结果 7组3T3-L1细胞油红O染色阳性面积、OD值、TG含量及脂联素和PPARγ的mRNA表达量差异均有统计学意义(P<0.05)。不同浓度BDE-47组的油红O染色阳性面积、OD值均高于阴性对照组(P<0.05)。18.75 μmol/L BDE-47组TG含量高于阴性对照组(P<0.05)。25、18.75、12.5、7.5 μmol/L BDE-47组和阳性对照组PPARγ 的mRNA相对表达量均高于阴性对照组(P<0.05);12.5 μmol/L BDE-47组 PPARγ 的mRNA相对表达量均高于25、18.75、7.5、2.5 µmol/L BDE-47组和阳性对照组(P<0.05)。12.5、7.5 μmol/L BDE-47组和阳性对照组脂联素的mRNA相对表达量均高于阴性对照组(P<0.05);12.5 µmol/L BDE-47组脂联素的mRNA相对表达量均高于25、18.75、2.5 µmol/L BDE-47组(P<0.05)。不同浓度BDE-47组的PPARγ和脂联素mRNA表达量近似倒“U”型分布。结论 BDE-47对小鼠3T3-L1细胞分化起促进作用,低浓度的BDE-47可能通过激活PPARγ活性诱导脂肪细胞分化。
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海且木汗·阿布杜热曼
阿依古丽·阿力木
李美艳
王永治
王嘉穗
刘早玲
关键词 肥胖2,2',4,4'-四溴联苯醚脂肪细胞分化过氧化物酶体增殖子激活受体γ    
AbstractObjective To investigate the effects of 2, 2', 4, 4'-tetrabromodiphenyl ether ( BDE-47 ) on the differentiation of mouse embryonic fibroblasts-3T3-L1, so as to provide the basis for revealing the mechanism of environmental obesity factors. Methods The 3T3-L1 cells were divided into five BDE-47 intervention groups ( 25, 18.75, 12.5, 7.5 and 2.5 µmol/L ), a positive control group (1 µmol/L 2, 4-thiazolidinedione) and a negative control group ( 0.1% dimethyl sulfoxide ) for the induction of differentiation. The lipid droplet accumulation in adipocytes was observed by oil red O staining treatment and detection of optical desity ( OD ) on the eighth day of differentiation. Triglyceride ( TG ) content was measured using the histiocyte TG enzymatic assay kit. The mRNA expression of adiponectin and peroxisome proliferator activated receptor-γ ( PPARγ ) was measured by RT-PCR. Results The positive areas of oil red O staining, OD values, TG content and mRNA expression of adiponectin and PPARγ in 3T3-L1 cells were significantly different among seven groups ( P<0.05 ). The positive areas of oil red O staining and OD values in the BDE-47 groups with different concentrations were higher than those in the negative control group ( P<0.05 ). The 18.75 µmol/L BDE-47 group had higher TG levels than the negative control group ( P<0.05 ). The mRNA expression of PPARγ in the 25, 18.75, 12.5, and 7.5 µmol/L BDE-47 groups and the positive control group was higher than that in the negative control group ( P<0.05 ). The mRNA expression of PPARγ in the 12.5 µmol/L BDE-47 group was higher than that in the 25, 18.75, 7.5, 2.5 µmol/L BDE-47 group and the positive control group ( P<0.05 ). The mRNA expression of adiponectin in the 12.5, 7.5 µmol/L BDE-47 group and the positive control group was higher than that in the negative control group ( P<0.05 ). The mRNA expression of adiponectin in the 12.5 µmol/L BDE-47 group was higher than that in the 25, 18.75, 2.5 µmol/L BDE-47 group ( P<0.05 ). The mRNA expression of PPARγ and adiponectin in the different concentration groups of BDE-47 distributed like inverted "U" shape. Conclusion BDE-47 can promote the differentiation of 3T3-L1 preadipocytes. Low concentration of BDE-47 may induce adipocyte differentiation by activating PPARγ.
Key wordsadiposity    2,2',4,4'-tetrabromodiphenyl ether    adipocyte differentiation    peroxisome proliferator-γ
收稿日期: 2021-01-04      修回日期: 2021-03-31     
中图分类号:  R114  
基金资助:新疆维吾尔自治区自然科学基金(2019D01C209)
作者简介: 海且木汗·阿布杜热曼,硕士在读
通信作者: 刘早玲,E-mail:zaolingliu@foxmail.com   
引用本文:   
海且木汗·阿布杜热曼, 阿依古丽·阿力木, 李美艳, 王永治, 王嘉穗, 刘早玲. 2,2',4,4'-四溴联苯醚对小鼠3T3-L1细胞分化的影响研究[J]. 预防医学, 2021, 33(6): 573-578.
Haiqiemuhan Abudureman, Ayiguli Alimu, LI Meiyan, WANG Yongzhi, WANG Jiasui, LIU Zaoling. Effects of 2,2',4,4' -tetrabromodiphenyl ether on differentiation of mouse 3T3-L1 cells. Preventive Medicine, 2021, 33(6): 573-578.
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[1] JUNG H O,FATIH K,JUNG I L,et al.Artemisia princeps inhibits adipogenic differentiation of 3T3-L1 pre-adipocytes via downregulation of PPARγ and MAPK pathways[J].Prev Nutr Food Sci,2019,24(3):299-307.
[2] FARMER S R.Regulation of PPAR gamma activity during adipogenesis[J]. Int J Obes(Lond),2005,29(Suppl.1):S13-S16.
[3] 马武仁,卿颖,李子琪,等.食品中典型持久性有机污染物暴露及毒性通路研究进展[J].中华预防医学杂志,2019,53(6):645-652.
[4] WISEMAN S B,WAN Y,CHANG H,et al.Polybrominated diphenyl ethers and their hydroxylated/methoxylated analogs: environmental sources, metabolic relationships, and relative toxicities[J].Mar Pollut Bull,2011,63(5/6/7/8/9/10/11/12):179-188.
[5] 翟金霞,童世庐.多溴联苯醚的健康效应研究进展[J].中华预防医学杂志,2016,50(6):559-562.
[6] SALES L B,KAMSTRA J H,CENIJN P H,et al.Effects of endocrine disrupting chemicals on in vitro global DNA methylation and adipocyte differentiation[J].Toxicology In Vitro,2013,27(6):1634-1643.
[7] 于雪. 利用MTT/MTS法和细胞成像技术评价fHMSN的载药性及AC对细胞增殖的影响[D].长春:吉林大学,2017.
[8] TUNG E W Y,BOUDREAU A,WADE M G,et al.Induction of adipocyte differentiation by polybrominated diphenyl ethers (PBDEs) in 3T3-L1 Cells[J/OL].PLoS One,2014,9(4):e94583. [2021-03-31].https://pubmed.ncbi.nlm.nih.gov/24722056/.DOI: 10.1371/journal.pone.0094583.
[9] KASSOTIS C,HOFFMAN K,STAPLETON H M.Characterization of adipogenic activity of house dust extracts and semi-volatile indoor contaminants in 3T3-L1 cells[J].Environ Sci Technol,2017,51(15):8735-8745.
[10] YANG C,ZHU L,KANG Q,et al.Chronic exposure to tetrabromodiphenyl ether (BDE-47) aggravates hepatic steatosis and liver fibrosis in diet-induced obese mice[J/OL].J Hazard Mater,2019,378:120766. [2021-03-31]. https://pubmed.ncbi.nlm.nih.gov/31226595/.DOI: 10.1016/j.jhazmat.2019.120766.
[11] MARION-LETELLIER R,SAVOYE G,GHOSH S.Fatty acids, eicosanoids and PPAR gamma[J].Eur J Pharmacol,2016,785:44-49.
[12] AL-GHADBAN S,DIAZ Z T,SINGER H J,et al.Increase in leptin and PPAR-γ gene expression in lipedema adipocytes differentiated in vitro from adipose-derived stem cells[J/OL].Cells,2020,9(2):430. [2021-03-31]. https://pubmed.ncbi.nlm.nih.gov/32059474/.DOI: 10.3390/cells9020430.
[13] WANG Y,WANG X H,LI R X.Interaction between peroxisome proliferator-activated receptor gamma polymorphism and overweight on diabetic retinopathy in a Chinese case-control study[J].Int J Clin Exp Med,2015,8(11):21647-21652.
[14] 宋琪,司婧,张蕴晖.孕期多溴联苯醚暴露与胎儿发育不良关联的研究进展[J].环境与职业医学,2017,34(12):1105-1110.
[15] FANG M L,WEBSTER T F,FERGUSON P L, et al.Characterizing the peroxisome proliferator-activated receptor (PPARγ) ligand binding potential of several major flame retardants, their metabolites, and chemical mixtures in house dust[J].Environ Health Perspect,2015,123(2):166-172.
[16] KAMSTRA J H,HRUBA E,BLUMBERS B,et al.Transcriptional and epigenetic mechanisms underlying enhanced in vitro adipocyte differentiation by the brominated flame retardant BDE-47[J].Environ Sci Technol,2014,48(7):4110-4119.
[17] 杨晓萌,周丽平,邓洁琳,等.脂联素降低右侧星状神经节活性抑制心肌梗死后室性心律失常[J].中华心律失常学杂志,2020,24(1):66-72.
[18] ISHTIAQ S M,RASHID H,HUSSAIN Z,et al.Adiponectin and PPAR: a setup for intricate crosstalk between obesity and non-alcoholic fatty liver disease[J].Rev Endocr Metab Disord,2019,20(3):253-261.
[19] SHERRIER M,LI H S.The impact of keto-adaptation on exercise performance and the role of metabolic-regulating cytokines[J].American J Clin Nutr,2019,110(3):562-573.
[20] YANG C X,WONG C M,WEI J T,et al.The brominated flame retardant BDE 47 upregulates purine metabolism and mitochondrial respiration to promote adipocyte differentiation[J].Sci Total Environ,2018,644:1312-1322.
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