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预防医学  2019, Vol. 31 Issue (3): 221-224    DOI: 10.19485/j.cnki.issn2096-5087.2019.03.002
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辽宁省外环境禽流感病毒监测结果
王璐璐, 孙海波, 孙佰红, 刘双, 宋亦春, 毛玲玲, 孙英伟, 姚文清
辽宁省疾病预防控制中心感染与传染性疾病防制所,辽宁 沈阳 110005
Surveillance for avian influenza virus in environments in Liaoning Province
WANG Lu-lu, SUN Hai-bo, SUN Bai-hong, LIU Shuang, SONG Yi-chun, MAO Ling-ling, SUN Ying-wei, YAO Wen-qing
Institute for Communicable and Infectious Diseases Prevention and Control,Liaoning Provincial Center for Disease Control and Prevention,Shenyang,Liaoning 110005,China
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摘要 目的 了解辽宁省外环境禽流感病毒分布特征,为预防和控制禽流感提供依据。方法 收集2016—2017年辽宁省城乡活禽市场、活禽批发市场、家禽规模养殖场(户)、散养户集中地和屠宰加工厂等场所禽流感病毒监测资料,以及2016年6—8月和2017年3—5月应急监测资料,采用实时荧光定量PCR法检测A型、H5、H7和H9亚型流感病毒核酸,分析辽宁省涉禽环境的禽流感病毒亚型分布。结果 2016—2017年采集并检测外环境标本4 037份,检出A型禽流感病毒阳性177份,阳性率为4.38%;2017年阳性率为6.26%,高于2016年的2.40%(P<0.05);以H9亚型阳性率最高,为3.07%;2017年首次检出H7亚型。第一至四季度禽流感病毒核酸阳性率分别为8.54%、4.88%、2.17%和1.45%。不同涉禽场所以活禽市场的禽流感病毒核酸阳性率最高,为8.08%,检出亚型以H9为主。不同类型涉禽标本以笼具表面擦拭物、清洗禽类的污水标本的禽流感病毒核酸阳性率较高,分别为23.47%和15.96%;各类标本均有H5、H9亚型检出,在禽类粪便、笼具表面擦拭物、禽类饮水和宰杀或摆放禽类砧板表面擦拭物中检测到H7亚型或其混合型。结论 辽宁省外环境禽流感病毒主要为H9和H5亚型,2017年检出H7亚型,活禽市场应重点监测和防控,特别是在冬春季。
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王璐璐
孙海波
孙佰红
刘双
宋亦春
毛玲玲
孙英伟
姚文清
关键词 禽流感外环境核酸检测活禽市场    
AbstractObjective To learn the epidemic distribution of avian influenza virus in external environments in Liaoning Province,and to provide evidence for the prevention and control of avian influenza.Methods The environmental samples were collected monthly during 2016 and 2017(including samples from emergency monitoring in June to August,2016 and March to May,2017)from live poultry markets,live poultry wholesale markets,large-scale poultry farms(households),free-range poultry famers and poultry processing factories in Liaoning Province. Real-time polymerase chain reaction assay was used to detect nucleic acid of Influenza A as well as H5,H7 and H9 subtypes in the environmental samples. The distribution of avian influenza virus in external environments in Liaoning Province was analyzed.Results A total of 4 037 environmental samples were collected and detected from 2016 to 2017,there were 177 copies of type A avian influenza virus and the positive rate of avian influenza A virus was 4.38%. The positive rate in 2017 was 6.26%, which was higher than 2.40% in 2016(P<0.05). H9 subtype had the highest positive rate of 3.07%;H7 subtype was first detected in 2017. The positive rates of avian influenza virus from the first to fourth quarters of a year were 8.54%,4.88%,2.17% and 1.45%,respectively. The positive rates of avian influenza virus in live poultry markets were 8.08%,the highest among different sites,and the subtypes were mainly H9. The positive rates of avian influenza virus in samples of poultry cage and poultry washing sewage were 23.47% and 15.96%. H5 and H9 subtypes were detected in all types of samples,and H7 subtypes or mixed types were detected in samples of feces,poultry cage,poultry drinking water and chopping board.Conclusion The subtypes of avian influenza virus in the environments of Liaoning Province were mostly H9 and H5,and the H7 was first detected in 2017. Live poultry markets should be the key monitoring sites,especially in winter and spring.
Key wordsAvian influenza    External environment    Nucleic acid detection    Poultry market
收稿日期: 2018-12-07      出版日期: 2019-03-04
ZTFLH:  R511.7  
通信作者: 姚文清,E-mail:wenqingyao@sina.com   
作者简介: 王璐璐,本科,主管医师,主要从事传染病防制工作
引用本文:   
王璐璐, 孙海波, 孙佰红, 刘双, 宋亦春, 毛玲玲, 孙英伟, 姚文清. 辽宁省外环境禽流感病毒监测结果[J]. 预防医学, 2019, 31(3): 221-224.
WANG Lu-lu, SUN Hai-bo, SUN Bai-hong, LIU Shuang, SONG Yi-chun, MAO Ling-ling, SUN Ying-wei, YAO Wen-qing. Surveillance for avian influenza virus in environments in Liaoning Province. Preventive Medicine, 2019, 31(3): 221-224.
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[1] 朱闻斐,舒跃龙. 新型H7N9禽流感病毒的病原学研究进展[J]. 生命科学,2015,27(5):518-524.
[2] 康来仪,董柏青,陈直平,等. 实用传染病防治[M]. 3版. 北京:学苑出版社,2010.
[3] BI Y H,ZHANG Z J,LIU W J,et al.Highly pathogenic avian influenza A(H5N1)virus struck migratory birds in China in 2015[J].Scientific Reports,2015,5:12986.
[4] GAO R,CAO B,HU Y,et al.Human infection with a novel avian-origin influenza A(H7N9)virus[J]. N Engl J Med,2013,368(20):1888-1897.
[5] SHI W,SHI Y,WU Y,et al.Origin and molecular characterization of the human-infecting H6N1 influenza virus in Taiwan[J]. Protein Cell,2013,4(11):846-853.
[6] CHEN H,YUAN H,GAO R,et al.Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection:a descriptive study[J]. Lancet,2014,383(9918):714-721.
[7] 张烨,李晓丹,邹淑梅,等. 2009—2013年我国活禽市场环境样本中禽流感病毒的检测[J]. 病毒学报,2015,31(6):615-619.
[8] 李保娣,李红育,张慧,等. 甘肃省2015—2016年外环境禽流感监测及职业暴露人群血清学分析[J]. 中国病毒病杂志,2017,7(4):267-270.
[9] 刘琳琳,叶国军,江永忠,等. 2013—2014年湖北省外环境中禽流感病毒污染情况分析[J]. 微生物与感染,2016,11(3):159-164.
[10] 刘美,刘晓青,傅伟杰,等. 2015—2016年江西省外环境禽流感病毒监测分析[J]. 现代预防医学,2017,44(23):4373-4376,4401.
[11] 郜振国,刘万里,赵俊,等. 2016年新疆地区外环境禽流感监测及职业暴露人群血清学调查[J]. 职业与健康,2018,34(8):1034-1037.
[12] 王笑笑,程伟,余昭,等. 浙江省冬春季涉禽场所禽流感病毒污染特征及影响因素研究[J]. 中华预防医学杂志,2016,50(3):250-254.
[13] 陈裕华,林鸿,彭南秀,等. 2015年广州市活禽批发市场市场链调查[J]. 中国动物检疫,2017,34(6):6-9.
[14] 邓立权,范明,黄飚,等. 吉林省活禽市场外环境禽流感污染情况调查[J]. 中国公共卫生,2017,33(3):493-495.
[15] 戴映雪,杜训波,杨磊,等. 冬春季节成都市活禽市场禽流感病毒污染情况及其影响因素[J]. 疾病监测,2017,32(2):135-140.
[16] 陈裕华,沈丹,朱建翠,等. 广东省广州市活禽批发市场从业人员H7N9流感“知信行”现状调查[J]. 中国动物检疫,2018,35(2):1-4.
[17] 曹家穗,富小飞,林云,等. 人感染H7N9禽流感农贸市场传播风险调查[J]. 浙江预防医学,2014,26(7):679-682.
[18] 何向莲,张翔,蒋小东,等. 不同人群人感染H7N9禽流感的知信行调查[J]. 浙江预防医学,2015,27(2):204-205,210.
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