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预防医学  2018, Vol. 30 Issue (12): 1212-1216    DOI: 10.19485/j.cnki.issn2096-5087.2018.12.006
  论著 本期目录 | 过刊浏览 | 高级检索 |
结核分枝杆菌耐多药相关蛋白筛选及诊断价值研究
潘稚芬1, 袁亚芳1, 2, 张君丽3, 张鹭3
1.嘉兴市第一医院结核科,浙江 嘉兴 314000;
2.蚌埠医学院;
3.复旦大学生命科学院
Screening and serodiagnosis potential of proteins related with multi-drug resistant Mycobacterium tuberculosis
PAN Zhi-fen*, YUAN Ya-fang, ZHANG Jun-li, ZHANG Lu
*The Tuberculosis Division of the First Hospital of Jiaxing,Jiaxing,Zhejiang 314000,China
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摘要 目的 筛选耐多药结核分枝杆菌菌体差异表达蛋白并分析其诊断耐药结核病的价值。方法 采用比较蛋白质组学技术,将临床分离的耐多药结核分枝杆菌菌体蛋白与标准株H37Rv进行比较,通过质谱分析和数据库鉴定差异表达蛋白点,在大肠杆菌中克隆、表达和纯化其中的NuoB(NADH脱氢酶I B链),采用ELISA法测定NuoB抗原在耐多药结核分枝杆菌感染患者、敏感结核分枝杆菌感染患者和正常对照者血清IgG抗体水平。结果 共发现重复性较好的耐多药结核分枝杆菌菌株差异蛋白点11个,经质谱分析鉴定出8个蛋白,其中NuoB在耐多药结核分枝杆菌中表达上调,并具有B细胞强结合表位,免疫原性较强。纯化获得的NuoB抗原在耐多药结核分枝杆菌感染患者血清中IgG阳性率为43.86%(25/57),在药物敏感结核分枝杆菌感染患者中为15.62%(5/32),在正常对照者中为10.00%(3/30),差异有统计学意义(P<0.05)。结论 NuoB抗原对耐多药结核病患者具有一定的区分度,具有耐多药结核病血清学鉴别诊断价值。
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潘稚芬
袁亚芳
张君丽
张鹭
关键词 耐多药结核病NuoB抗原蛋白质组学血清学诊断    
AbstractObjective To explore the differentially expressed proteins in multi-drug resistant(MDR)strains of Mycobacterium tuberculosis(TB)and their potential for serodiagnosis. Methods Differentially expressed proteins were detected from clinical isolates of MDR TB by protomic technology,compared with standard strain H37Rv. The interested protein spots were identified by mass spectrometry and database searching. The target antigen NuoB was cloned and purified from Escherichia coli. Its IgG levels induced from MDR TB patients,drug-sensitive TB patients and healthy people were detected by enzyme-linked immuno sorbent assay(ELISA).Results Totally 11 differentially expressed proteins in MDR TB strains were detected and 8 proteins were identified by mass spectrometry. NuoB showed both higher expression level in clinical MDR strains and stronger B cell epitope. The serum IgG positive rate of purified NuoB was 44% in MDR TB patients,which was higher than that in drug-sensitive TB patients(15.62%)and healthy subjects(10.00%,P<0.05).Conclusion NuoB protein can be a useful diagnostic marker in MDR TB.
Key wordsmulti-drug resistant tuberculosis    NuoB    Proteome    Serodiagnosis
          出版日期: 2018-12-04
中图分类号:  R521  
基金资助:浙江省卫生和计划生育委员会医药卫生科技计划项目(2016KYB288); 嘉兴市科技计划项目(2018AD32089); 嘉兴市医学重点学科呼吸科项目(04-Z-11)
通信作者: 潘稚芬,E-mail:597085662@qq.com   
作者简介: 潘稚芬,本科,主任医师,主要从事结核病临床诊治管理工作
引用本文:   
潘稚芬, 袁亚芳, 张君丽, 张鹭. 结核分枝杆菌耐多药相关蛋白筛选及诊断价值研究[J]. 预防医学, 2018, 30(12): 1212-1216.
PAN Zhi-fen, YUAN Ya-fang, ZHANG Jun-li, ZHANG Lu. Screening and serodiagnosis potential of proteins related with multi-drug resistant Mycobacterium tuberculosis. Preventive Medicine, 2018, 30(12): 1212-1216.
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http://www.zjyfyxzz.com/CN/10.19485/j.cnki.issn2096-5087.2018.12.006      或      http://www.zjyfyxzz.com/CN/Y2018/V30/I12/1212
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