Please wait a minute...
文章检索
预防医学  2017, Vol. 29 Issue (10): 983-986    DOI: 10.19485/j.cnki.issn1007-0931.2017.10.003
  论著 本期目录 | 过刊浏览 | 高级检索 |
经皮染毒反复束缚对SD大鼠生长的影响
黄雅丽,黎关龙,来伟旗,陈炜,徐娟,朱勇,陈琼姜,陈日萍,张佳莉,洪雅青,张芳芳,金锋,顾刘金
浙江省医学科学院,浙江 杭州 310013
A study on the growth effects of SD rats by repeated restraint in dermal exposure test
HUANG Ya-li,LI Guan-long,LAI Wei-qi,CHEN Wei,XU Juan,ZHU Yong,CHEN Qiong-jiang,CHEN Ri-ping,ZHANG Jia-li,HONG Ya-qing,ZHANG Fang-fang,JIN Feng,GU Liu-jin
Zhejiang Academy of Medical Sciences,Hangzhou,Zhejiang,310013,China
全文: PDF(512 KB)  
输出: BibTeX | EndNote (RIS)      
摘要 目的 分析经皮染毒反复束缚对大鼠生长的影响。方法 8周龄SD大鼠随机分为束缚组和对照组,每组20只,雌雄各半。束缚组大鼠模拟经皮染毒试验每天束缚固定6 h,连续91 d,对照组不作上述处理。观察两组大鼠临床症状、体重和摄食量变化,试验结束时进行血液学、血清生化和尿液生化检测,并作大体解剖和病理组织学检查,采用广义估计方程比较两组间差异。结果 广义估计方程统计结果显示,无论雌雄大鼠,体重变化组别和试验时间存在交互作用(P<0.05),且束缚组雄性大鼠体重低于对照组(P<0.05)。进一步分析发现,雄性大鼠体重从试验第4周开始低于对照组(P<0.05),并且组别与试验时间间出现交互作用(P<0.05);雌性大鼠从试验第8周开始,组别与试验时间间出现交互作用(P<0.05)。束缚组雌性大鼠红细胞(RBC)、血红蛋白(HGB)、血容积比(HCT)和雄性大鼠RBC和HGB水平均高于对照组(P<0.05),但无生物学意义;两组间其他观察指标差异均无统计学意义(P>0.05)。 结论 经皮染毒反复束缚影响大鼠体重的增长,且雄性大鼠的敏感性高于雌性大鼠。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
黄雅丽
黎关龙
来伟旗
陈炜
徐娟
朱勇
陈琼姜
陈日萍
张佳莉
洪雅青
张芳芳
金锋
顾刘金
关键词 经皮染毒束缚生长广义估计方程    
AbstractObjective To analysis the effects on the growth of rats by repeated restraint in dermal exposure test. Methods SD rats in the restraint group was bound for 6 hours per day for 91 days according the way by dermal exposure,while SD rats in the control group didn't receive the treatment. Clinical signs,body weight and food consumption changes were observed for 91 days. When the study was terminated,hematology,clinical biochemistry,urinalyses,gross necropsy,and histopathology were carried out. Statistical methods such as the generalized estimating equation were used to compare the differences between two groups. Results The statistical results of generalized estimating equation showed that there was an interaction between the group and test time for male and female rats in body weight changes(P<0.05),and the body weight of male rats in the restraint group was lower than the control group(P<0.05). Further analysis showed that for male rats there was significant difference between groups since the forth week(P<0.05),and the interaction was found between groups and test time(P<0.05). For female rats,the interaction was found since the eighth week between the group and test time(P<0.05).There was no significant differences in other parameters between two groups(P>0.05). Conclusion Repeated restraint during dermal exposure affected the body weight gain of rats,and the sensitivity of male rats was higher than that of female rats.
Key wordsDermal exposure    Restraint    Growth    Generalized estimating equation
收稿日期: 2017-05-04      出版日期: 2017-10-10
ZTFLH:  R33  
基金资助:浙江省科技厅科技条件建设项目(2013F10052)
通信作者: 顾刘金,E-mail:Guliujin@163.com   
作者简介: 黄雅丽,硕士,助理研究员,主要从事毒理学研究工作
引用本文:   
黄雅丽,黎关龙,来伟旗,陈炜,徐娟,朱勇,陈琼姜,陈日萍,张佳莉,洪雅青,张芳芳,金锋,顾刘金. 经皮染毒反复束缚对SD大鼠生长的影响[J]. 预防医学, 2017, 29(10): 983-986.
HUANG Ya-li,LI Guan-long,LAI Wei-qi,CHEN Wei,XU Juan,ZHU Yong,CHEN Qiong-jiang,CHEN Ri-ping,ZHANG Jia-li,HONG Ya-qing,ZHANG Fang-fang,JIN Feng,GU Liu-jin. A study on the growth effects of SD rats by repeated restraint in dermal exposure test. Preventive Medicine, 2017, 29(10): 983-986.
链接本文:  
http://www.zjyfyxzz.com/CN/10.19485/j.cnki.issn1007-0931.2017.10.003      或      http://www.zjyfyxzz.com/CN/Y2017/V29/I10/983
[1] RYU H J,SEO M Y,JUNG S K,et al. Zinc oxide nanoparticles:a 90-day repeated-dose dermal toxicity study in rats[J] . Int J Nanomedicine,2014,9(Suppl 2):137-144.
[2] 田璐,魏金锋,王爱平. 经皮给药实验应注意的问题[J] . 中国药理学与毒理学杂志,2013,27(3):516.
[3] RYU H J,SEONG N W,SO B J,et al. Evaluation of silica nanoparticle toxicity after topical exposure for 90 days[J] . Int J Nanomedicine,2014,9(Suppl 2):127-136.
[4] BREGLIA R,BUI Q,BURNETT D,et al. A 13-week dermal repeat-dose neurotoxicity study of hydrodesulfurized kerosene in rats[J] . Int J Toxicol,2014,33(Suppl 1):68S-77S.
[5] SUREKHA P,KISHORE A S,SRINIVAS A,et al. Repeated dose dermal toxicity study of nano zinc oxide with Sprague-Dawley rats[J] . Cutan Ocul Toxicol,2012,31(1):26-32.
[6] KIM M J,KWACK S J,LIM S K,et al. Toxicological evaluation of isopropylparaben and isobutylparaben mixture in Sprague-Dawley rats following 28 days of dermal exposure[J] . Regul Toxicol Pharmacol,2015,73(2):544-551.
[7] 孟真,陈苘,吴惠岭,等. 二氯甲烷重复剂量经皮染毒对大鼠毒作用的研究[J] . 浙江预防医学,2016,28(3):234- 239.
[8] 中华人民共和国环境保护部化学品登记中心. 化学品测试方法(健康效应卷)[M] . 2版. 北京:中国环境出版社,2013:71-81
[9] 孙振球,徐勇勇. 医学统计学[M] . 3版. 北京:人民卫生出版社,2012.
[10] 张文彤,童伟. SPSS统计高级教程[M] . 2版. 北京:高等教育出版社,2013.
[11] 洪雅青,顾刘金,黄雅丽,等. 不同经口给药方式对 SD 大鼠生长的影响[J] . 浙江预防医学,2014,26(8):861- 864.
[1] 周玉润, 林颖, 孙建乐, 陈洋. 温州市3 625例早产儿体格生长状况分析[J]. 预防医学, 2017, 29(7): 740-744.
[2] 张浩永, 章宏欣. 乳腺癌手术前后EGFR和VEGF表达及意义[J]. 预防医学, 2017, 29(7): 744-747.
[3] 李绍波, 陈丽丽, 赖小英, 金小红, 王昕昕. 特异性免疫治疗对哮喘大鼠肺组织RORγtmRNA表达的影响[J]. 预防医学, 2017, 29(5): 482-484.
[4] 许霞,陈丹青,邱丽倩. 巨大儿和正常体重儿0~3岁生长发育比较[J]. 预防医学, 2017, 29(12): 1273-1275.
[5] 高何刚,杜赛,王瑞,王晶. 超高效液相色谱-串联质谱法同时测定水果中杀菌剂和植物生长调节剂[J]. 预防医学, 2017, 29(10): 1074-1077.
[6] 柴灵莺,陈科,李华,张颖,谢胡咪娜,尚佳,田维政,杨萍. 强化乳铁蛋白配方奶粉对婴儿体格及红细胞指数的影响[J]. 预防医学, 2016, 28(7): 682-686.
[7] 黄佳敏, 张艳, 吕时铭, 王青青. 血清胰岛素和IGFBP3水平与子宫内膜癌相关性研究[J]. 预防医学, 2016, 28(4): 358-361.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed