Abstract：Objective To analysis the effects on the growth of rats by repeated restraint in dermal exposure test. Methods SD rats in the restraint group was bound for 6 hours per day for 91 days according the way by dermal exposure，while SD rats in the control group didn't receive the treatment. Clinical signs，body weight and food consumption changes were observed for 91 days. When the study was terminated，hematology，clinical biochemistry，urinalyses，gross necropsy，and histopathology were carried out. Statistical methods such as the generalized estimating equation were used to compare the differences between two groups. Results The statistical results of generalized estimating equation showed that there was an interaction between the group and test time for male and female rats in body weight changes（P<0.05），and the body weight of male rats in the restraint group was lower than the control group（P<0.05）. Further analysis showed that for male rats there was significant difference between groups since the forth week（P<0.05），and the interaction was found between groups and test time（P<0.05）. For female rats，the interaction was found since the eighth week between the group and test time（P<0.05）.There was no significant differences in other parameters between two groups（P>0.05）. Conclusion Repeated restraint during dermal exposure affected the body weight gain of rats，and the sensitivity of male rats was higher than that of female rats.
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