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预防医学  2017, Vol. 29 Issue (7): 694-698    DOI: 10.19485/j.cnki.issn1007-0931.2017.07.010
  疾病预防与控制 本期目录 | 过刊浏览 | 高级检索 |
舟山渔场海洋鱼类异尖线虫感染调查及分子鉴定
周晶耀, 林启, 张辉, 张均和, 顾舟霞
舟山市疾病预防控制中心,浙江 舟山 316021
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摘要 目的了解舟山渔场海洋鱼类异尖线虫感染状况。方法从舟山市农贸市场购买带鱼、小黄鱼和鲐鱼3个品种89尾鱼,解剖后在光学显微镜下观察异尖线虫幼虫形态并进行初步鉴定;采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)方法分析核糖体DNA(rDNA)内转录间隔区(ITS)进行分子学鉴定。结果在82尾鱼中检出异尖线虫幼虫,感染率为92.13%,共获得1 023条异尖线虫样品。鱼小肠、肠系膜和性腺(62.35%)以及体腔周围脏器(37.05%)是主要的寄生部位。随机选择435 条异尖线虫进行PCR-RFLP分子鉴定,结果显示有异尖属和宫脂属2个属的7种异尖线虫,包括简单异尖线虫(A. simplex s.s. ,占4.60%)、派氏异尖线虫(A. pegreffii,占81.61%)、典型异尖线虫(A. typica,占0.23%)、内弯宫脂线虫(H. aduncum,占2.07%)、费氏宫脂线虫(H. fabri,占3.91%)、宫脂线虫未定种(H. spp,占2.99%)和厦门宫脂线虫(H. amoyense 4.37%)。结论舟山渔场带鱼、小黄鱼和鲐鱼异尖线虫感染情况较为严重,派氏异尖线虫为感染优势种。
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周晶耀
林启
张辉
张均和
顾舟霞
关键词 舟山渔场异尖线虫分子鉴定    
收稿日期: 2016-10-18      出版日期: 2017-11-15
ZTFLH:  R383.1  
基金资助:舟山市科技计划项目(2012C13034)
通讯作者: 林启,E-mail:77648098@qq.com   
作者简介: 周晶耀,本科,主管技师,主要从事微生物检验工作
引用本文:   
周晶耀, 林启, 张辉, 张均和, 顾舟霞. 舟山渔场海洋鱼类异尖线虫感染调查及分子鉴定[J]. 预防医学, 2017, 29(7): 694-698.
链接本文:  
http://www.zjyfyxzz.com/CN/Y2017/V29/I7/694
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